Xiong K G, Ke K Y, Chen L F, Wei D H, Lin X Q, Chen L
Fuzhou Municipal Infectious Disease Hospital, Infectious Disease Hospital, Fujian Medical University, Fuzhou 350025, China.
Zhonghua Gan Zang Bing Za Zhi. 2017 Apr 20;25(4):263-267. doi: 10.3760/cma.j.issn.1007-3418.2017.04.006.
To investigate the expression of programmed death-1 (PD-1) in liver tissue and its association with liver pathology in patients with autoimmune hepatitis (AIH). A total of 54 AIH patients (38 in the active stage and 16 in the remission stage) were enrolled, and 9 healthy volunteers were enrolled as control group. Immunohistochemistry combined with quantitative image analysis was used to measure the expression of PD-1 in liver tissue. The t-test, rank sum test, one-way analysis of variance, least significant difference t-test, Mann-Whitney U test, and Pearson relation analysis were used for statistical analysis of different types of data. The AIH group had a significantly higher positive rate of PD-1 in liver tissue than the control group (13.57%±6.84% vs 2.22%±0.66%, < 0.01), and the patients in the active stage of AIH had a significantly higher positive rate of PD-1 in liver tissue than those in the remission stage (16.53%±7.72% vs 6.56%±3.16%, < 0.01). The positive rate of PD-1 in liver tissue was 6.56%±3.16% in G0 group, 14.33%±5.08% in G1-2 group, and 19.23%±5.41% in G3-4 group ( < 0.01), but there was no significant difference in the positive rate of PD-1 between S0, S1-2, and S3-4 groups ( > 0.05). In AIH patients, the positive rate of PD-1 in liver tissue was positively correlated with the levels of total bilirubin, alanine aminotransferase, aspartate aminotransferase, and IgG ( = 0.665, 0.721, 0.711, and 0.813, all < 0.01). AIH patients have regulated PD-1 expression in liver tissue, which is closely associated with liver inflammation and is not associated with fibrosis degree, suggesting that PD-1 is involved in the development and progression of inflammation in AIH patients.
探讨程序性死亡因子1（PD-1）在自身免疫性肝炎（AIH）患者肝组织中的表达及其与肝脏病理的关系。共纳入54例AIH患者（活动期38例，缓解期16例），并纳入9名健康志愿者作为对照组。采用免疫组织化学结合定量图像分析方法检测肝组织中PD-1的表达。对不同类型的数据采用t检验、秩和检验、单因素方差分析、最小显著差t检验、Mann-Whitney U检验和Pearson相关性分析进行统计学分析。AIH组肝组织中PD-1阳性率显著高于对照组（13.57%±6.84% vs 2.22%±0.66%，P<0.01），AIH活动期患者肝组织中PD-1阳性率显著高于缓解期患者（16.53%±7.72% vs 6.56%±3.16%，P<0.01）。G0组肝组织中PD-1阳性率为6.56%±3.16%，G1-2组为14.33%±5.08%，G3-4组为19.23%±5.41%（P<0.01），但S0、S1-2和S3-4组之间PD-1阳性率差异无统计学意义（P>0.05）。在AIH患者中，肝组织中PD-1阳性率与总胆红素、丙氨酸氨基转移酶、天冬氨酸氨基转移酶和IgG水平呈正相关（r分别为0.665、0.721、0.711和0.813，均P<0.01）。AIH患者肝组织中PD-1表达存在调控，其与肝脏炎症密切相关，与纤维化程度无关，提示PD-1参与了AIH患者炎症的发生发展。
