He Yu Xuan, Zhang Hong, Li Hong Yan, Zhang Yong, Jia Qi Peng, Li Zong Shuai, Zhao Xing Xu
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
Department of Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, Gansu, China; Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Lanzhou 730000, Gansu, China.
Biomed Environ Sci. 2017 Apr;30(4):301-307. doi: 10.3967/bes2017.040.
In the present study, we used a proteomics approach based on a two-dimensional electrophoresis (2-DE) reference map to investigate protein expression in the ovarian tissues of pubertal Swiss-Webster mice subjected to carbon ion radiation (CIR). Among the identified proteins, ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) is associated with the cell cycle[1] and that it influences proliferation in ovarian tissues. We analyzed the expression of UCH-L1 and the proliferation marker proliferation cell nuclear antigen (PCNA) following CIR using immunoblotting and immunofluorescence. The proteomics and biochemical results provide insight into the underlying mechanisms of CIR toxicity in ovarian tissues.
在本研究中，我们采用基于二维电泳（2-DE）参考图谱的蛋白质组学方法，来研究遭受碳离子辐射（CIR）的青春期瑞士 Webster 小鼠卵巢组织中的蛋白质表达情况。在鉴定出的蛋白质中，泛素羧基末端水解酶 L1（UCH-L1）与细胞周期相关[1]，并且它会影响卵巢组织中的增殖。我们使用免疫印迹和免疫荧光分析了 CIR 后 UCH-L1 和增殖标志物增殖细胞核抗原（PCNA）的表达。蛋白质组学和生化结果为深入了解卵巢组织中 CIR 毒性的潜在机制提供了线索。
