Maniquet Adrien, Bruyer Nicolas, Raffin Guy, Baco-Antionali Franck, Demesmay Claire, Dugas Vincent, Randon Jérôme
IFP Énergies nouvelles - établissement de Lyon, Expérimentation Procédés, Rond-point de l'échangeur de Solaize, BP3, 69360 Solaize, France.
Univ Lyon, CNRS, Université Claude Bernard Lyon 1, Ens de Lyon, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.
J Chromatogr A. 2017 Jun 30;1504:105-111. doi: 10.1016/j.chroma.2017.05.002. Epub 2017 May 2.
80% vinyltrimethoxysilane-based hybrid silica monoliths (80-VTMS), which have been initially developed for separation in reversed-phase liquid chromatography, have been investigated in high pressure gas chromatography separations (carrier gas pressure up to 60bar) and compared to silica monolithic columns. The behavior of both silica and 80-VTMS monolithic columns was investigated using helium, nitrogen and carbon dioxide as carrier gas. The efficiency of 80-VTMS monolithic columns was shown to vary differently than silica monolithic columns according to the temperature and the carrier gas used. Carrier gas nature was a significant parameter on the retention for both silica and vinyl columns in relation to its adsorption onto the stationary phase in such high pressure conditions. The comparison of retention and selectivity between 80-VTMS monoliths and silica was performed under helium using the logarithm of the retention factor according to the number of carbon atoms combined to Kovats indexes. The very good performances of these columns were demonstrated, allowing the separation of 8 compounds in less than 1min.
最初为反相液相色谱分离而开发的80%乙烯基三甲氧基硅烷基杂化硅胶整体柱（80-VTMS），已在高压气相色谱分离（载气压力高达60巴）中进行了研究，并与硅胶整体柱进行了比较。使用氦气、氮气和二氧化碳作为载气，研究了硅胶和80-VTMS整体柱的性能。结果表明，80-VTMS整体柱的效率根据温度和所用载气的不同，与硅胶整体柱的变化方式不同。在如此高压条件下，载气性质对于硅胶柱和乙烯基柱的保留而言，是一个与固定相吸附相关的重要参数。在氦气条件下，根据与科瓦茨指数结合的碳原子数，使用保留因子的对数对80-VTMS整体柱和硅胶之间的保留和选择性进行了比较。这些色谱柱表现出非常好的性能，能够在不到1分钟的时间内分离8种化合物。
