Hedemann U, Schürmann M, Schwinger E
Institut für Humangenetik der Medizinischen Universität zu Lübeck, Federal Republic of Germany.
Genome. 1988 Oct;30(5):652-5. doi: 10.1139/g88-110.
Human metaphase chromosomes, fixed on slides, have beent treated with 8 different restriction endonucleases and 29 combinations of 2 restriction enzymes prior to staining with Giemsa. The endonucleases AluI and DdeI and the combinations AluI + DdeI, AluI + HaeIII, AluI + HinfI, and AluI + MboI have then been used to digest metaphase chromosomes of nine individuals with C-band variants of chromosomes 1 or 9, obtained by the CBG technique. The restriction enzyme resistant chromatin of the paracentromeric regions of chromosomes 1 and 9 has been measured and compared with the corresponding CBG-bands. The size of the enzyme resistant chromatin regions depend upon the type of enzyme(s) used. Treatment with AluI + MboI was the only digestion that acted differently on different chromosome pairs. However, within one pair of homologous chromosomes, all digestions revealed the same variations as conventional C-banding.
固定在载玻片上的人类中期染色体，在用吉姆萨染色之前，先用8种不同的限制性内切酶和29种两种限制性酶的组合进行了处理。然后，使用限制性内切酶AluI和DdeI以及AluI + DdeI、AluI + HaeIII、AluI + HinfI和AluI + MboI组合，对通过CBG技术获得的9个具有1号或9号染色体C带变异的个体的中期染色体进行消化。测量了1号和9号染色体着丝粒旁区域的限制性酶抗性染色质，并与相应的CBG带进行了比较。酶抗性染色质区域的大小取决于所用酶的类型。用AluI + MboI处理是唯一对不同染色体对作用不同的消化方式。然而，在一对同源染色体内，所有消化都显示出与传统C带相同的变异。
