Yohai L I, Bianchi N O
Instituto Multidisciplinario de Biologia Celular, IMBICE, La Plata, Argentina.
Hereditas. 1993;118(3):265-71. doi: 10.1111/j.1601-5223.1993.00265.x.
C and Re-banding chromosome heteromorphisms were analysed in blood cultures from 43 normal individuals. Restriction enzymes used were AluI, DdeI, HaeIII, and MboI. Chromosome pairs exhibiting heteromorphisms were: 1, 3, 4, 6, 9, 10, 12-16, and 18-22. Each individual showed a specific combination of C- and Re-banding heteromorphisms not shared by any other individual in the series. Some polymorphisms could be detected by all the banding methods used. Others could be detected by some of the banding methods, and in some cases by only one of the banding methods used. The efficiency of each banding method to detect chromosomal polymorphisms depended on the type of polymorphism and the chromosomal pair analysed. Our results indicate that Re-banding polymorphisms occur due to changes in base composition of different fractions of heterochromatin or due to the presence or absence of different heterochromatic subsets. C- and Re-banding are complementary methods that expand the identification of chromosomal markers and which can be used to identify the parental origin of individual chromosomes.
对43名正常个体血培养物中的C带和再显带染色体异态性进行了分析。使用的限制性内切酶有AluI、DdeI、HaeIII和MboI。呈现异态性的染色体对为：1、3、4、6、9、10、12 - 16以及18 - 22。每个个体都显示出一种特定的C带和再显带异态性组合，该系列中的其他个体均未共享。一些多态性可通过所有使用的显带方法检测到。其他的可通过某些显带方法检测到，在某些情况下仅能通过所使用的一种显带方法检测到。每种显带方法检测染色体多态性的效率取决于多态性的类型和所分析的染色体对。我们的结果表明，再显带多态性是由于异染色质不同部分碱基组成的变化或由于不同异染色质亚群的存在或缺失所致。C带和再显带是互补方法，可扩展染色体标记的识别，可用于识别单个染色体的亲本来源。
