Amin Bahareh, Hosseini Samira, Hosseinzadeh Hossein
Cellular and Molecular Research Center,Department of Physiology and Pharmacology, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzever, Iran.
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Pharm Res. 2017 Winter;16(1):187-200.
The aim of this study was to evaluate the anti-nociceptive effects of a low, sub-effective dose of amitriptyline, in combination with the different doses of ethanolic and aqueous extracts of Crocus sativus following sciatic nerve chronic constriction injury (CCI) in rats. Amitriptyline (3, 10 and 30 mg/Kg, i.p.) and the extracts (25, 50 and 100 mg/Kg, i.p.), were separately administered at the time of CCI for 7 consecutive days. In combination therapy, the sub-antinociceptive dose of amitriptyline (3 mg/Kg) was given with the three different doses of extracts for seven days. Mechanical allodynia, thermal allodynia and thermal hyperalgesia were evaluated by von Frey, acetone and radiant heat tests, respectively, 1 day before and on days 3, 5 and 7 after surgery. Co-administration of amitriptyline (3 mg/Kg) with aqueous extract (50, 100 mg/Kg,) produced more potent cold anti-allodynic (P < 0.01 and P < 0.001, respectively) as well as thermal anti-hyperalgesic (P < 0.05) effects than that produced by each of them. Amitriptyline (3 mg/Kg) plus ethanolic extract (50, 100 mg/Kg) produced more potent cold anti-allodynic (P < 0.05 and P < 0.001, respectively) as well as thermal anti-hyperalgesic (P < 0.05) effects as compared with the sum effects produced by each of them. Mechanical anti-allodynia effect was only potentiated with the co-administration of amitriptyline with the high dose of aqueous extract (100 mg/Kg, P < 0.001). Our study supports the use of saffron as an adjunctive to amitriptyline to improve the therapeutic outcome in the management of neuropathic pain.
本研究的目的是评估低剂量、低于有效剂量的阿米替林，与不同剂量的藏红花乙醇提取物和水提取物联合使用时，对大鼠坐骨神经慢性压迫损伤（CCI）后的抗伤害感受作用。在CCI时，连续7天分别腹腔注射阿米替林（3、10和30mg/Kg）和提取物（25、50和100mg/Kg）。在联合治疗中，将亚抗伤害感受剂量的阿米替林（3mg/Kg）与三种不同剂量的提取物一起给药7天。分别在手术前1天以及手术后第3、5和7天，通过von Frey、丙酮和辐射热试验评估机械性异常性疼痛、冷异常性疼痛和热痛觉过敏。与单独使用时相比，阿米替林（3mg/Kg）与水提取物（50、100mg/Kg）联合使用产生了更强的冷抗异常性疼痛作用（分别为P < 0.01和P < 0.001）以及热抗痛觉过敏作用（P < 0.05）。与单独使用时的总效应相比，阿米替林（3mg/Kg）加乙醇提取物（50、100mg/Kg）产生了更强的冷抗异常性疼痛作用（分别为P < 0.05和P < 0.001）以及热抗痛觉过敏作用（P < 0.05）。仅在阿米替林与高剂量水提取物（100mg/Kg）联合使用时，机械性抗异常性疼痛作用才增强（P < 0.001）。我们的研究支持将藏红花作为阿米替林的辅助药物，以改善神经性疼痛管理的治疗效果。
