Andreis Sabrina N, Perreten Vincent, Schwendener Sybille
Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
mSphere. 2017 May 3;2(3). doi: 10.1128/mSphere.00117-17. eCollection 2017 May-Jun.
Whole-genome sequencing of penicillin-resistant strain SAN1670 from bovine mastitis milk revealed a novel β-lactamase operon consisting of the β-lactamase-encoding gene , the antirepressor-encoding gene , and the repressor-encoding gene . The functionality of was demonstrated by gene expression in . The operon was chromosomally located in SAN1670 and present in 10 additional unrelated strains, suggesting intrinsic penicillin resistance in . Furthermore, a GenBank search revealed more unique potential β-lactamases in species. Penicillins are an important group of antibiotics used to treat various types of infections caused by Gram-positive bacteria. So far, the gene was the only known β-lactamase gene in staphylococci. However, other putative β-lactamases were identified, and one of them was shown to be a novel functional β-lactamase encoded by in , further limiting treatment options.
对来自患乳腺炎奶牛乳汁的耐青霉素菌株SAN1670进行全基因组测序,发现了一个新的β-内酰胺酶操纵子,它由β-内酰胺酶编码基因、抗阻遏物编码基因和阻遏物编码基因组成。通过在……中的基因表达证明了……的功能。该操纵子位于SAN1670的染色体上,并且在另外10株不相关的菌株中也存在,这表明……中存在内在的青霉素抗性。此外,通过GenBank搜索发现……物种中存在更多独特的潜在β-内酰胺酶。青霉素是用于治疗由革兰氏阳性菌引起的各种感染的一类重要抗生素。到目前为止,……基因是葡萄球菌中唯一已知的β-内酰胺酶基因。然而,还鉴定出了其他推定的β-内酰胺酶,其中之一被证明是由……中的……编码的一种新型功能性β-内酰胺酶,这进一步限制了治疗选择。