Balasubramani Sundararajan, Rajendhiran Thamaraiselvi, Moola Anil Kumar, Diana Ranjitha Kumari Bollipo
Department of Plant Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620 024, India.
Environ Sci Pollut Res Int. 2017 Jun;24(17):15125-15133. doi: 10.1007/s11356-017-9118-y. Epub 2017 May 11.
It is believed that nanoemulsions were emerged as a promising candidate to improve the qualities of natural essential oil towards antimicrobial and insecticidal applications. In the present study, we have focused on the encapsulation of Vitex negundo L. leaf essential oil using Polysorbate80 for its different biological activities including antioxidant, bactericidal and larvicidal activity against dengue fever vector Aedes aegypti L. Initially, the nanoemulsion was prepared by low energy method and droplet size of the formulated nanoemulsion was characterized by using Dynamic Light Scattering analysis. The freshly prepared V. negundo essential nanoemulsion was observed with the mean droplet size of below 200 nm indicating its excellent stability. Further, the larvicidal activity of essential oil and nanoemulsion with various concentrations (25, 50, 100, 200 and 400 ppm). The larvicidal activities were tested 2nd and 3rd instar larval mortality rate that was observed against the 12 and 24 h exposure period. After a 12 h exposure period, the larvicidal activities of 2nd instar larva were observed as essential oil (73.33 ± 1.88), nanoemulsion (81.00 ± 0.88) and the larvicidal activities of 3rd instar larva were displayed essential oil (70.33 ± 2.60) and nanoemulsion (79.00 ± 3.70). Likewise, after a 24 h exposure period, the larvicidal activities of 2nd instar larva were observed as essential oil (90.30 ± 2.15), nanoemulsion (94.33 ± 1.20) and the larvicidal activities of 3rd instar larva were essential oil (80.66 ± 0.66) and nanoemulsion (93.00 ± 1.25) respectively. We finally concluded that the developed plant-based emulsion essential oil systems were thermodynamically stable. Owing to its improved bioavailability and biocompatibility, formulated nanoemulsion can be used in various biomedical applications including drug delivery as well as disease transmitting mosquito vector control. Graphical abstract ᅟ.
据信，纳米乳液已成为一种有前途的候选物，可用于改善天然精油在抗菌和杀虫应用方面的品质。在本研究中，我们专注于使用聚山梨醇酯80包封黄荆叶精油，以发挥其不同的生物活性，包括抗氧化、杀菌以及对登革热媒介埃及伊蚊的杀幼虫活性。最初，通过低能法制备纳米乳液，并使用动态光散射分析对所制备纳米乳液的液滴大小进行表征。观察到新制备的黄荆精油纳米乳液的平均液滴大小低于200nm，表明其具有出色的稳定性。此外，还测试了不同浓度（25、50、100、200和400ppm）的精油和纳米乳液的杀幼虫活性。在12小时和24小时的暴露期后，观察了第二龄和第三龄幼虫的死亡率以测试杀幼虫活性。在暴露12小时后，观察到第二龄幼虫的杀幼虫活性：精油为（73.33±1.88），纳米乳液为（81.00±0.88）；第三龄幼虫的杀幼虫活性：精油为（70.33±2.60），纳米乳液为（79.00±3.70）。同样，在暴露24小时后，观察到第二龄幼虫的杀幼虫活性：精油为（90.30±2.15），纳米乳液为（94.33±1.20）；第三龄幼虫的杀幼虫活性：精油为（80.66±0.66），纳米乳液为（93.00±1.25）。我们最终得出结论，所开发的基于植物的乳液精油体系具有热力学稳定性。由于其提高的生物利用度和生物相容性，所制备的纳米乳液可用于各种生物医学应用，包括药物递送以及疾病传播蚊媒控制。图形摘要ᅟ
