Gosselet Fabien
Université Artois, EA 2465, laboratoire de la Barrière Hémato-Encéphalique (LBHE), rue Jean Souvraz, SP18, F-62300 Lens, France.
Med Sci (Paris). 2017 Apr;33(4):423-431. doi: 10.1051/medsci/20173304013. Epub 2017 May 12.
The blood-brain barrier (BBB) is located at the brain microvessel level and isolates the brain from the whole body, thus restricting molecule and cell exchanges between cerebral and peripheral compartments. In order to better decipher and understand the BBB physiology and development, and to investigate transport mechanism and toxicity of neuropharmaceuticals, several in vitro BBB models have been developed using animal or human cells, primary or immortalized cells. The aim of this review is to explain to the reader the major criteria required for a pertinent in vitro BBB model and to briefly expose the different models currently available with their characteristics with a special focus on the static models.
血脑屏障（BBB）位于脑微血管水平，将大脑与全身隔离开来，从而限制大脑与外周区域之间的分子和细胞交换。为了更好地解读和理解血脑屏障的生理学和发育过程，并研究神经药物的转运机制和毒性，人们利用动物或人类细胞、原代细胞或永生化细胞开发了几种体外血脑屏障模型。这篇综述的目的是向读者解释建立一个合适的体外血脑屏障模型所需的主要标准，并简要介绍目前可用的不同模型及其特点，特别关注静态模型。
