Ma Yan, Chu Junjie, Ma Jianjun, Ning Lei, Zhou Ke, Fang Xiangqian
Department of Orthopedic Surgery, Sir Run Run Shaw Institute of Clinical Medicine, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang 310016, P.R. China.
Department of Head and Neck Surgery, Sir Run Run Shaw Institute of Clinical Medicine, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang 310016, P.R. China.
Mol Med Rep. 2017 Jul;16(1):288-294. doi: 10.3892/mmr.2017.6574. Epub 2017 May 11.
Natural compounds are alternative agents that have therapeutic potential for preventing and treating osteoporosis. Traditionally, sanguinarine has been used clinically due to its diverse biological properties, including antimicrobial, anti‑inflammatory and anticancer effects. Recently, for the first time, it was reported that sanguinarine inhibits osteoclast differentiation and bone resorption by suppressing the tumor necrosis factor ligand superfamily member 11‑induced nuclear factor‑κB and extracellular signal‑regulated kinase signaling pathways in vitro. Therefore, the present study further investigated the pharmacological effect of sanguinarine on osteoporosis in vivo. Micro‑computed tomography and histomorphometry analysis demonstrated that sanguinarine, at low and high concentrations, prevents ovariectomy (OVX)‑induced bone loss. In addition, further investigation of the cellular response in vivo revealed that sanguinarine inhibited osteoclastic bone resorption and promoted osteoblastic bone formation in a dose‑dependent manner. Therefore, the present study demonstrated that sanguinarine protected mice from OVX‑induced osteoporosis by modulating bone remodeling, indicating that sanguinarine may have potential in the treatment of osteoporosis.
天然化合物是具有预防和治疗骨质疏松症治疗潜力的替代药物。传统上，血根碱因其多种生物学特性，包括抗菌、抗炎和抗癌作用而被临床使用。最近，首次报道血根碱在体外通过抑制肿瘤坏死因子配体超家族成员11诱导的核因子-κB和细胞外信号调节激酶信号通路来抑制破骨细胞分化和骨吸收。因此，本研究进一步研究了血根碱在体内对骨质疏松症的药理作用。微计算机断层扫描和组织形态计量学分析表明，低浓度和高浓度的血根碱均可预防去卵巢（OVX）诱导的骨质流失。此外，对体内细胞反应的进一步研究表明，血根碱以剂量依赖的方式抑制破骨细胞骨吸收并促进成骨细胞骨形成。因此，本研究表明，血根碱通过调节骨重塑保护小鼠免受OVX诱导的骨质疏松症，表明血根碱在骨质疏松症治疗中可能具有潜力。
