主要组织相容性（B）复合体单倍型B23、B24和B30对劳斯肉瘤的反应

Anti-Rous sarcoma response of major histocompatibility (B) complex haplotypes B23, B24 and B30.

作者信息

Taylor R L, Clare R A, Ward P H, Briles R W, Briles W E

机构信息

Department of Animal and Nutritional Sciences, University of New Hampshire, Durham 03824.

出版信息

Anim Genet. 1988;19(3):277-84. doi: 10.1111/j.1365-2052.1988.tb00816.x.

DOI:10.1111/j.1365-2052.1988.tb00816.x

PMID:2849889

Abstract

Responses to Rous sarcoma virus (RSV) induced tumours were studied in UNH 105, a non-inbred line of New Hampshire chickens. Six single male matings encompassing a total of 50 dams produced 345 progeny which segregated for B complex genotypes B23/B23, B23/B24, B23/B30, B24/B24, B24/B30 and B30/B30. Six-week-old chicks were wingweb inoculated with a pseudotype of Bryan high titre Rous sarcoma virus, BH RSV (RAV-1). Tumours were scored for size six times over a 10-week period post-inoculation. Each chick was assigned a tumour profile index (TPI) as an indicator of immunological response. The number of days to death (DTD) was recorded for 148 chicks with terminal tumours. Genotypes B23/B23, B23/B24 and B23/B30, with TPIs of 1.8, 1.7 and 2.0 respectively, did not differ significantly from each other, suggesting dominance of response of B23 over B24 and B30 haplotypes. B24/B30 chicks with the highest TPI (3.4) and shortest DTD (34.6) were significantly different from B30/B30 (2.8; 41.6) but not from B24/B24 (3.1; 34.9) suggesting dominance of response of the B24 haplotype over B30 in the absence of B23.

摘要

在新罕布什尔鸡的非近交系UNH 105中研究了对劳氏肉瘤病毒（RSV）诱导肿瘤的反应。6次单雄交配，共涉及50只母禽，产生了345只后代，这些后代按B复合基因型B23/B23、B23/B24、B23/B30、B24/B24、B24/B30和B30/B30进行分离。6周龄的雏鸡通过翼蹼接种布赖恩高滴度劳氏肉瘤病毒假型，即BH RSV（RAV-1）。在接种后10周内对肿瘤大小进行了6次评分。为每只雏鸡指定一个肿瘤概况指数（TPI）作为免疫反应的指标。记录了148只患有晚期肿瘤雏鸡的死亡天数（DTD）。基因型为B23/B23、B23/B24和B23/B30的雏鸡，其TPI分别为1.8、1.7和2.0，彼此之间无显著差异，表明B23对B24和B30单倍型的反应具有优势。TPI最高（3.4）且DTD最短（34.6）的B24/B30雏鸡与B30/B30雏鸡（2.8；41.6）有显著差异，但与B24/B24雏鸡（3.1；34.9）无显著差异，这表明在不存在B23的情况下，B24单倍型对B30的反应具有优势。