Gusterson B A, Machin L G, Gullick W J, Gibbs N M, Powles T J, Elliott C, Ashley S, Monaghan P, Harrison S
Institute of Cancer Research, Haddow Laboratories, Surrey, UK.
Br J Cancer. 1988 Oct;58(4):453-7. doi: 10.1038/bjc.1988.239.
An antibody, 21N, raised against a synthetic peptide from the predicted sequence of the c-erbB-2 protein has been used immunocytochemically in a retrospective study of formalin fixed paraffin embedded breast biopsies. Fourteen out of 103 infiltrating ductal carcinomas exhibited positive membrane staining. Fifty-four of these tumours had lymph node involvement of which nine contained stained cells. These were all cases where the primary tumour was positive. In this series there was no correlation between c-erbB-2 overexpression and lymph node status. In five of the positive cases studied there was an associated in situ component which was also positively stained. Ten out of 24 pure intraduct carcinomas showed membrane staining, but none of the 149 benign conditions studied, which included 22 radial scars and 13 cases of atypical ductal proliferation, demonstrated the pattern of staining associated with overexpression. It is concluded that the c-erbB-2 protein is overexpressed in a minority (approximately 14%) of infiltrating ductal carcinomas and only in cells that are cytologically malignant. Overexpression of c-erbB-2 is considered in relation to pathogenesis.
一种针对c-erbB-2蛋白预测序列的合成肽产生的抗体21N，已被用于对福尔马林固定石蜡包埋的乳腺活检组织进行免疫细胞化学回顾性研究。103例浸润性导管癌中有14例显示细胞膜染色阳性。其中54例肿瘤有淋巴结受累，9例含有染色细胞。这些都是原发性肿瘤呈阳性的病例。在该系列研究中，c-erbB-2过表达与淋巴结状态之间没有相关性。在研究的5例阳性病例中，有原位成分且也呈阳性染色。24例纯导管内癌中有10例显示细胞膜染色，但在研究的149例良性病变中，包括22例放射状瘢痕和13例非典型导管增生，均未显示与过表达相关的染色模式。得出的结论是，c-erbB-2蛋白在少数（约14%）浸润性导管癌中过表达，且仅在细胞学上呈恶性的细胞中过表达。c-erbB-2的过表达与发病机制有关。
