Fundación Instituto Leloir, IIBBA-CONICET, Buenos Aires, Argentina.
Photochem Photobiol. 2017 May;93(3):656-665. doi: 10.1111/php.12762.
Light signals trigger precise changes in gene expression networks that activate distinctive developmental programs in plants. The transcriptome is shaped at different stages, both by the regulation of gene expression and also by posttranscriptional mechanisms that alter the sequence or abundance of the transcripts generated. Posttranscriptional mechanisms have attracted much interest in recent years with the advent of high-throughput technologies and bioinformatics tools. One such posttranscriptional process, alternative splicing, increases proteome diversity without increasing gene number by changing the function of individual proteins, while another, miRNA-mediated gene silencing, fine-tunes the amount of mRNA produced. The manner in which plants make use of these two crucial posttranscriptional mechanisms to respond to light and adapt to their environment is the focus of active research. In this review, we summarize the current knowledge of light-mediated posttranscriptional control in Arabidopsis thaliana and focus on the biological impact of the various posttranscriptional processes. We also discuss a potential cross talk between the alternative splicing and miRNA pathways, highlighting the complexity of light responsiveness.
光信号触发精确的基因表达网络变化，从而激活植物中独特的发育程序。转录组在不同阶段都受到基因表达调控以及通过改变产生的转录本序列或丰度的转录后机制的影响。随着高通量技术和生物信息学工具的出现，转录后机制近年来引起了广泛关注。一种转录后过程——选择性剪接，通过改变单个蛋白质的功能，在不增加基因数量的情况下增加蛋白质组的多样性，而另一种——miRNA 介导的基因沉默，则精细调节产生的 mRNA 量。植物利用这两种关键的转录后机制来响应光并适应环境的方式是当前研究的重点。在这篇综述中，我们总结了目前对拟南芥中光介导的转录后控制的认识，并重点介绍了各种转录后过程的生物学影响。我们还讨论了选择性剪接和 miRNA 途径之间的潜在串扰，突出了光反应的复杂性。
