Zhang Qiao, Hu Xiaolong, Hui Fuhai, Song Qi, Cui Can, Wang Changli, Zhao Qingchun
Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang 110840, China; Department of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China.
Biomed Pharmacother. 2017 Jul;91:812-822. doi: 10.1016/j.biopha.2017.04.131. Epub 2017 May 10.
Alpinia oxyphylla Miq. (A. oxyphylla), as a kind of medicine which also be used as food, is widely used in East Asian for the treatment of dyspepsia, diarrhea, abdominal pain and deficiency cold of spleen and stomach. This study aimed to investigate the protective effects of ethanol extract (EE) and its dichloromethane fraction (DM) of A. oxyphylla, which are rich in phenolic compounds, against CCl-induced hepatic injury in vitro and in vivo. EE, DM and silymarin ameliorated CCl-induced decrease of cell viability and increase of reactive oxygen species (ROS) in HepG2 cells. The CCl-induced changes of glutathione (GSH) and methane dicarboxylic aldehyde (MDA) levels, and the decrease of superoxide dismutase (SOD) and catalase (CAT) activities were all restored with the pretreatment of EE, DM and silymarin. The results in liver injury model in rats showed that EE, DM and silymarin could significant decrease the levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and total bilirubin than the model group. Liver histopathology revealed that EE and DM attenuated the incidence of liver lesions triggered by CCl intoxication. They also effectively relieved CCl-induced oxidative damage. Western blot analysis indicated NF-E2-related factor (Nrf2) pathway played an critical role in the protection of EE and DM against CCl-induced oxidative stress. In conclusion, the extracts from A. oxyphylla might be used as hepatoprotective agents.
益智（Alpinia oxyphylla Miq.）是一种药食两用的植物，在东亚地区被广泛用于治疗消化不良、腹泻、腹痛以及脾胃虚寒。本研究旨在探究富含酚类化合物的益智乙醇提取物（EE）及其二氯甲烷馏分（DM）在体外和体内对四氯化碳（CCl）诱导的肝损伤的保护作用。EE、DM和水飞蓟素改善了CCl诱导的HepG2细胞活力下降和活性氧（ROS）增加的情况。EE、DM和水飞蓟素预处理后，CCl诱导的谷胱甘肽（GSH）和丙二醛（MDA）水平变化以及超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性下降均得到恢复。大鼠肝损伤模型的结果表明，与模型组相比，EE、DM和水飞蓟素可显著降低血清天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）和总胆红素水平。肝脏组织病理学显示，EE和DM减轻了CCl中毒引发的肝脏病变发生率。它们还有效缓解了CCl诱导的氧化损伤。蛋白质印迹分析表明，核因子E2相关因子（Nrf2）通路在EE和DM对CCl诱导的氧化应激的保护中起关键作用。总之，益智提取物可能用作肝脏保护剂。
