Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin 300134, China.
Talanta. 2017 Aug 1;170:74-80. doi: 10.1016/j.talanta.2017.02.040. Epub 2017 Feb 21.
A new fluorescent sensing platform based on ultrathin metal-organic framework (MOF) nanosheets (MnDMS) was prepared from the flexible ligand 2,2-dimethylsuccinate and Mn ions. The MnDMS nanoparticles can be obtained by simply ultrasonication of the MnDMS crystal, and then can be exfoliated into nanosheets by Li-intercalation method. The MnDMS nanosheets can be easily assembled with biological probes, leading to efficient fluorescence quenching of the fluorophore tagged ssDNA and microRNA (miRNA). By using a hybridization chain reaction (HCR) strategy, the fluorescence signal can be obviously amplified. A good linearity was obtained from 1 pM to 200 pM of target ssDNA, with a detection limit of 0.2 pM. The HCR/MnDMS system provides an effective way to monitor miRNA in living cells. Therefore, the MnDMS nanosheets can be used as a new kind of platform in biomedical sensing applications.
一种基于超薄金属-有机骨架（MOF）纳米片（MnDMS）的新型荧光传感平台由柔性配体 2,2-二甲基琥珀酸和 Mn 离子制备而成。MnDMS 纳米片可通过 MnDMS 晶体的简单超声处理获得，然后通过 Li 插层法剥离成纳米片。MnDMS 纳米片可与生物探针轻松组装，导致标记 ssDNA 和 microRNA（miRNA）的荧光团有效猝灭。通过杂交链式反应（HCR）策略，荧光信号可明显放大。对于目标 ssDNA 的 1 pM 至 200 pM 范围，获得了良好的线性关系，检测限为 0.2 pM。HCR/MnDMS 系统为监测活细胞中的 miRNA 提供了一种有效的方法。因此，MnDMS 纳米片可用作生物医学传感应用中的新型平台。
