倍他米松会导致雄性大鼠出现代际生殖损伤。

Betamethasone causes intergenerational reproductive impairment in male rats.

作者信息

Borges Cibele Dos Santos, Pacheco Taina Louise, da Silva Katiussia Pinho, Fernandes Fábio Henrique, Gregory Mary, Pupo André Sampaio, Salvadori Daisy Maria F, Cyr Daniel G, Kempinas Wilma De G

机构信息

Department of Morphology, Institute of Biosciences of Botucatu, UNESP - São Paulo State University, Distrito de Rubião Junior s/nº, Botucatu, SP 18618-689, Brazil.

出版信息

Reprod Toxicol. 2017 Aug;71:108-117. doi: 10.1016/j.reprotox.2017.04.012. Epub 2017 May 10.

DOI:10.1016/j.reprotox.2017.04.012

PMID:28501545

Abstract

Prenatal betamethasone (BM) exposure in rats negatively impacts sperm quality and male fertility. Studies have shown that BM can cause multi-generational effects on the pituitary-adrenal-axis of rats. The objective of this study was to assess the reproductive development and fertility of male rats (F2) whose fathers (F1) were exposed to BM (0.1mg/kg) on gestational days 12, 13, 18 and 19. In F2 rats, there was a significant reduction in body weights of the BM-treated group at PND 1 as well as delayed onset of puberty, and decreases in FSH levels, Leydig cell volume, sperm number and motility, seminal vesicle contractility and ejaculated volume. Furthermore, increased serum LH levels, sperm DNA damage and abnormal morphology were observed, resulting in reduced fertility. In conclusion, prenatal BM-treatment leads to intergenerational long-term reproductive impairment in male rats, raising concern regarding the widespread use of BM in preterm births.

摘要

孕期大鼠暴露于倍他米松（BM）会对精子质量和雄性生育能力产生负面影响。研究表明，BM可对大鼠的垂体-肾上腺轴产生多代影响。本研究的目的是评估其父亲（F1）在妊娠第12、13、18和19天暴露于BM（0.1mg/kg）的雄性大鼠（F2）的生殖发育和生育能力。在F2大鼠中，BM处理组在出生后第1天体重显著降低，青春期开始延迟，促卵泡生成素（FSH）水平、睾丸间质细胞体积、精子数量和活力、精囊收缩性和射精量均下降。此外，观察到血清促黄体生成素（LH）水平升高、精子DNA损伤和形态异常，导致生育能力降低。总之，产前BM处理会导致雄性大鼠跨代长期生殖功能受损，这引发了人们对BM在早产中广泛使用的担忧。