Tagliabue Giovanni, Faoro Valentina, Rizzo Serena, Sblattero Daniele, Saccani Andrea, Riccio Gabriele, Bellini Tommaso, Salina Matteo, Buscaglia Marco, Marcello Alessandro
Proxentia S.r.l., 20139 Milano, Italy.
Molecular Virology Laboratory, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.
Biochem Biophys Res Commun. 2017 Oct 28;492(4):558-564. doi: 10.1016/j.bbrc.2017.05.025. Epub 2017 May 10.
Flaviviruses are widespread and cause clinically relevant arboviral diseases that impact locally and as imported travel-related infections. Direct detection of viraemia is limited, being typically undetectable at onset of symptoms. Therefore, diagnosis is primarily based on serology, which is complicated by high cross-reactivity across different species. The overlapping geographical distribution of the vectors in areas with a weak healthcare system, the increase of international travel and the similarity of symptoms highlight the need for rapid and reliable multi-parametric diagnostic tests in point-of-care formats. To this end we developed a bi-parametric serological microarray using recombinant NS1 proteins from Tick-borne encephalitis virus and West Nile virus coupled to a low-cost, label-free detection device based on the Reflective Phantom Interface (RPI) principle. Specific sequential detection of antibodies in solution demonstrates the feasibility of the approach for the surveillance and diagnosis of Flaviviruses.
黄病毒广泛传播，可引发具有临床相关性的虫媒病毒疾病，这些疾病在当地造成影响，同时也作为与旅行相关的输入性感染存在。病毒血症的直接检测存在局限性，在症状出现时通常无法检测到。因此，诊断主要基于血清学，但不同物种间的高交叉反应性使血清学诊断变得复杂。在医疗保健系统薄弱地区，病媒的地理分布重叠，国际旅行增加以及症状相似，凸显了在即时护理模式下开展快速且可靠的多参数诊断检测的必要性。为此，我们开发了一种双参数血清学微阵列，该微阵列使用来自蜱传脑炎病毒和西尼罗河病毒的重组NS1蛋白，并与基于反射幻影接口（RPI）原理的低成本、无标记检测设备相结合。溶液中抗体的特异性顺序检测证明了该方法用于黄病毒监测和诊断的可行性。
