Hu Huan-Xin, Du Fang-Ying, Fu Wei-Wei, Jiang Shan-Feng, Cao Jin, Xu Shen-Hui, Wang Hui-Ping, Chang Hui, Goswami Nandu, Gao Yun-Fang
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an, China.
Department of Genetics and Molecular Biology, School of Medicine, Xi'an Jiaotong University, Xi'an, China.
J Comp Physiol B. 2017 Jul;187(5-6):869-879. doi: 10.1007/s00360-017-1092-7. Epub 2017 May 13.
We compared the effects of hibernation inactivity and 14-day hindlimb unloading in non-hibernating period on biochemical, rheological, and hematological parameters of blood in Daurian ground squirrels (Spermophilus dauricus). Twenty-four squirrels were randomly divided into four groups: control (CON), hibernation (HIB), post-hibernation (POST), and 14-day hindlimb unloading (HU). The results showed that serum enzymes (L-lactate dehydrogenase, alanine aminotransferase, and aspartate aminotransferase) activities decreased in HIB, POST, and HU squirrels compared with CON. Total protein (including albumin and globulin) maintained in HIB but decreased in HU compared with CON. Total cholesterol and high-density lipoprotein-cholesterol increased in HIB but maintained in HU and POST compared with CON. Meanwhile, serum creatinine decreased and urea increased in HU compared with CON. All blood ions concentrations were unchanged in HIB, POST, and HU squirrels compared with CON except calcium which increased in HIB compared with CON, and phosphorus which increased in HIB and POST compared with CON. Most of detected serum biochemical analytes in POST recovered to the CON level. Blood viscosity, which was unchanged in all shear rates in HU, increased in HIB and recovered in POST in lower shear rates compared with CON. Erythrocyte and corpuscular volume decreased in HIB and HU but maintained in POST compared with CON. All the routine hematological parameters recovered in POST as compared with CON except platelet, which decreased in HIB and POST but maintained in HU compared with CON. In conclusion, our results suggested a remarkable ability to maintain blood homeostasis in hibernating squirrels.
我们比较了冬眠期的静止状态以及非冬眠期14天的后肢卸载对达乌尔黄鼠（达乌尔黄鼠）血液生化、流变学和血液学参数的影响。24只黄鼠被随机分为四组：对照组（CON）、冬眠组（HIB）、冬眠后组（POST）和14天的后肢卸载组（HU）。结果表明，与CON组相比，HIB组、POST组和HU组的血清酶（L-乳酸脱氢酶、丙氨酸氨基转移酶和天冬氨酸氨基转移酶）活性降低。总蛋白（包括白蛋白和球蛋白）在HIB组保持不变，但与CON组相比，HU组降低。与CON组相比，HIB组的总胆固醇和高密度脂蛋白胆固醇增加，但HU组和POST组保持不变。同时，与CON组相比，HU组的血清肌酐降低，尿素增加。与CON组相比，HIB组、POST组和HU组的所有血液离子浓度均未发生变化，不过与CON组相比，HIB组的钙增加，HIB组和POST组的磷增加。POST组中检测到的大多数血清生化分析物恢复到CON组水平。HU组在所有剪切速率下血液粘度均未发生变化，与CON组相比，HIB组血液粘度增加，POST组在较低剪切速率下恢复。与CON组相比，HIB组和HU组的红细胞和细胞体积减小，但POST组保持不变。与CON组相比，POST组所有常规血液学参数均恢复，不过血小板除外，与CON组相比，HIB组和POST组血小板减少，但HU组保持不变。总之，我们的结果表明冬眠黄鼠具有维持血液内稳态的显著能力。
