Harbison Amanda M, Nguyen Jenny Ngoc Tran
Northern Virginia Community College, Manassas Campus, 6901 Sudley Road, Manassas, VA, 20109, USA.
Methods Mol Biol. 2017;1606:193-203. doi: 10.1007/978-1-4939-6990-6_13.
Polymerase chain reaction (PCR) enables the amplification of a specific sequence of deoxyribonucleic acid (DNA) through the process of three main steps: template DNA denaturation, annealing of the primers to complementary sequences, and primer extension to synthesize DNA strands. By using this method, the target sequence will be copied and amplified at an exponential rate. PCR provides a qualitative method for identifying DNA from fresh or dried cells/body fluids, formalin-fixed archival tissue specimens, and ancient specimens.Herein we describe basic information for performing successful PCR experiments using the amplification of a human Alu insertion on the PV92 gene locus on chromosome 16 as an example method.
聚合酶链反应（PCR）通过三个主要步骤实现脱氧核糖核酸（DNA）特定序列的扩增：模板DNA变性、引物与互补序列退火以及引物延伸以合成DNA链。通过使用这种方法，目标序列将以指数速率被复制和扩增。PCR为从新鲜或干燥的细胞/体液、福尔马林固定的存档组织标本以及古代标本中鉴定DNA提供了一种定性方法。在此，我们以扩增16号染色体上PV92基因座的人类Alu插入序列为例，描述进行成功PCR实验的基本信息。
