用于代谢组学的液相色谱-质谱联用技术

LC-Mass Spectrometry for Metabolomics.

作者信息

Dailey Allyson L

机构信息

Department of Chemistry and Biochemistry, George Mason University, 10920 George Mason Circle, MS1A9, Manassas, VA, 20110, USA.

出版信息

Methods Mol Biol. 2017;1606:333-340. doi: 10.1007/978-1-4939-6990-6_21.

DOI:10.1007/978-1-4939-6990-6_21

PMID:28502010

Abstract

The field of metabolomics is greatly being refined by the addition of new technologies. LC-MS has allowed researchers to explore additional metabolites which were not originally captured through GC-MS. Through the customizability of the LC columns and mass spectrometer, it is now easier to tailor the instrument to your research needs. Herein, we describe a protocol for sample preparation and data acquisition for a global metabolomic analysis of tissues or feces.

摘要

代谢组学领域正通过新技术的加入而得到极大的完善。液相色谱 - 质谱联用技术使研究人员能够探索那些最初通过气相色谱 - 质谱联用技术未捕获到的其他代谢物。通过液相色谱柱和质谱仪的可定制性，现在更容易根据研究需求来定制仪器。在此，我们描述了一种用于组织或粪便的全球代谢组学分析的样品制备和数据采集方案。