[ABIN1不参与伊马替尼上调A20以抑制Jurkat T细胞中NF-κB途径的激活]

[ABIN1 is not involved in imatinib upregulating A20 to inhibit the activation of NF-κB pathway in Jurkat T cells].

作者信息

Chen Qian, Wang Senlin, Lin Chen, Chen Shaohua, Zhao Xiaoling, Li Yangqiu

机构信息

Department of Microbiology and Immunology, Medical College, Jinan University, Guangzhou 510632, China.

Department of Microbiology and Immunology, Medical College, Jinan University, Guangzhou 510632, China. *Corresponding author, E-mail:

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017 May;33(5):577-580.

PMID:28502291

Abstract

Objective To investigate the effect of imatinib (IM) on the expressions of A20-binding inhibitor of NF-κB1 (ABIN1) and A20 in Jurkat T cells. Methods Jurkat T cells were treated with 25, 50 and 100 nmol/L IM for 24 hours. The mRNA and protein levels of ABIN1, A20 and NF-κB were detected by real-time quantitative PCR and Western blotting. Results IM significantly inhibited both mRNA and protein levels of ABIN1 and NF-κB, but raised the mRNA and protein levels of A20; while phorbol 12-myristate 13-acetate/ionomycin increased the expression levels of ABIN1 and A20 mRNA and protein. Conclusion IM could upregulate A20 protein to inhibit the activation of NF-κB pathway in Jurkat T cells, which was independent of the ABIN1 protein.

摘要

目的探讨伊马替尼（IM）对Jurkat T细胞中NF-κB1的A20结合抑制剂（ABIN1）和A20表达的影响。方法用25、50和100 nmol/L的IM处理Jurkat T细胞24小时。通过实时定量PCR和蛋白质印迹法检测ABIN1、A20和NF-κB的mRNA和蛋白质水平。结果 IM显著抑制ABIN1和NF-κB的mRNA和蛋白质水平，但提高A20的mRNA和蛋白质水平；而佛波醇12-肉豆蔻酸酯13-乙酸酯/离子霉素增加ABIN1和A20 mRNA及蛋白质的表达水平。结论 IM可上调A20蛋白以抑制Jurkat T细胞中NF-κB途径的激活，这与ABIN1蛋白无关。