Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, Gansu 730070, China; The Phytochemistry Key Laboratory of Tibetan Plateau of Qinghai Province, College of Pharmacy, Qinghai Nationalities University, Xining, Qinghai 810007, China.
Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, Gansu 730070, China.
Anal Chim Acta. 2017 Jun 22;973:34-42. doi: 10.1016/j.aca.2017.03.041. Epub 2017 Apr 10.
Graphitic carbon nitride quantum dots (g-CNQDs) are rarely used in the field of electrochemiluminescence. In this paper, g-CNQDs have a strong and stable electrochemiluminescence (ECL) signal generated in the presence of co-reactant KSO. The ECL signal of g-CNQDs was quenched by the mechanism of resonance energy transfer (RET) between donor g-CNQDs and receptor riboflavin (RF) that is proved by UV-vis absorption spectroscopy, electrochemiluminescence and fluorescence emission spectroscopy analysis technology. Therefore, we achieved detection of the riboflavin content in the drug tablets of vitamin B using ECL and FL. The determination results of ECL showed that the riboflavin content of the drug vitamin B (VB) tablets was consistent with the fluorescence (FL) analysis, with wider linear range of 0.02-11 μM and lower minimum detection limit of 0.63 nM (S/N = 3) than FL. Hence, the riboflavin content in human serum was further detected using ECL. The relative standard deviation is less than 6.5%, with an acceptable recovery of 95.33%-104.22%, which means that this sensor has potential applications in the actual sample analysis. As a new ECL luminary, g-CNQDs have opened a new field for the development and application of ECL sensor.
石墨相氮化碳量子点（g-CNQDs）在电化学发光领域的应用较少。本文中，g-CNQDs 在共存反应物 KSO 的存在下产生强而稳定的电化学发光（ECL）信号。通过紫外-可见吸收光谱、电化学发光和荧光发射光谱分析技术证明，g-CNQDs 的 ECL 信号被供体 g-CNQDs 和受体核黄素（RF）之间的共振能量转移（RET）机制猝灭。因此，我们实现了使用 ECL 和 FL 对药物维生素 B 片中核黄素含量的检测。ECL 的测定结果表明，药物维生素 B（VB）片中的核黄素含量与荧光（FL）分析一致，与 FL 相比，其线性范围更宽（0.02-11 μM），检测限更低（6.3 nM，S/N = 3）。因此，进一步使用 ECL 检测了人血清中的核黄素含量。相对标准偏差小于 6.5%，回收率为 95.33%-104.22%，表明该传感器在实际样品分析中具有潜在的应用价值。作为一种新型的 ECL 发光体，g-CNQDs 为 ECL 传感器的开发和应用开辟了新的领域。
