College of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, China.
College of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, China.
Talanta. 2023 Jan 15;252:123886. doi: 10.1016/j.talanta.2022.123886. Epub 2022 Aug 28.
An ultrasensitive resonance energy transfer based "on-off" switch electrochemiluminescence (ECL) immunosensing strategy was proposed and applied for prostate specific antigen (PSA) detection. In detail, self-enhancing nanohybrids of nano-CeO and AuNPs decorated graphitic carbon nitride quantum dots (CeO-AuNPs-g-CNQDs) were synthesized, which produced high ECL emission with coreactant KSO for "switch-on" state. Noticeably, due to the reversible Ce↔ Ce reaction, nano-CeO could serve as highly-efficient coreaction accelerator and induce the decomposition of SO into SO, which endowed the g-CNQDs/KSO system with enhanced ECL emission. Moreover, AuNPs acted crucial role in promoting the electronic transmission rate and providing sites to immobilize the biomolecules. On the other hand, the AgNPs-VB nanocomposites were used as dual labels to quench the ECL signal of g-CNQDs-KSO system via ECL-RET, leading to the decreased ECL for "switch-off" state. On the basis of sandwich-typed immunosensing strategy, the developed ECL immunosensing platform exhibited sensitive response to PSA in a linear range of 1.0 × 10 50 ng mL with a low detection limit of 0.0045 pg mL (S/N = 3). This work provided a promising tool in early clinical diagnostics of prostate cancer.
基于超灵敏共振能量转移的“开-关”开关电化学发光（ECL）免疫传感策略被提出并应用于前列腺特异性抗原（PSA）检测。具体而言，合成了纳米 CeO 和 AuNPs 修饰的石墨相氮化碳量子点（CeO-AuNPs-g-CNQDs）自增强纳米杂化物，其与反应物 KSO 产生高 ECL 发射，处于“开”状态。值得注意的是，由于 Ce↔Ce 反应是可逆的，纳米 CeO 可以作为高效的反应加速剂，并诱导 SO 分解为 SO，从而使 g-CNQDs/KSO 体系具有增强的 ECL 发射。此外，AuNPs 在促进电子传输速率和提供固定生物分子的位点方面发挥着至关重要的作用。另一方面，AgNPs-VB 纳米复合材料被用作双重标记物，通过 ECL-RET 猝灭 g-CNQDs-KSO 体系的 ECL 信号，导致“关”状态下 ECL 降低。基于三明治型免疫传感策略，所开发的 ECL 免疫传感平台对 PSA 表现出敏感的响应，线性范围为 1.0×10 50 ng mL，检测限低至 0.0045 pg mL（S/N=3）。这项工作为前列腺癌的早期临床诊断提供了一种有前途的工具。
