Miura Yusuke, Furukawa Takayuki, Kobayashi Miho, Shrestha Rojeet, Takahashi Ryoji, Shimizu Chikara, Chiba Hitoshi, Hui Shu-Ping
Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, Japan.
Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Kita-14, Nishi-5, Kita-ku, Sapporo 060-8648, Japan.
Steroids. 2017 Jul;123:43-49. doi: 10.1016/j.steroids.2017.05.003. Epub 2017 May 11.
Urine has been utilized as a source of biomarkers in renal disease. However, urinary lipids have not attracted much attention so far. Here we studied urinary cholesteryl ester (CE) and its relevance in renal disease.
Quantitative analysis of CE molecular species in serum, urinary supernatant, and urinary sediment from patients with renal disease (N=64) and non-renal disease (N=23) was carried out using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and deuterated CEs as internal standards.
Validation study showed good precision and accuracy of LC-MS/MS. Many CE species were detected in the urinary sediment and supernatant in the renal disease group, whereas only a few CE species were detected in the other group. In the renal disease group, the sum of the concentrations of all CE species showed a significant correlation between the sediment and the supernatant from urinary samples (r=0.876, p<0.001); however, the composition of CEs was significantly different between them. Further, the composition of CEs of the supernatant was similar to that of the serum.
Our LC-MS/MS analysis uncovered a distinct CE profile in urinary sediment from patients with renal disease, suggesting a possible contribution of CEs in urothelial cells to the development of renal disease.
尿液已被用作肾脏疾病生物标志物的来源。然而，尿脂质迄今为止尚未引起太多关注。在此，我们研究了尿胆固醇酯（CE）及其在肾脏疾病中的相关性。
使用液相色谱 - 串联质谱法（LC-MS/MS）并以氘代CE作为内标，对肾病患者（N = 64）和非肾病患者（N = 23）的血清、尿上清液和尿沉渣中的CE分子种类进行定量分析。
验证研究表明LC-MS/MS具有良好的精密度和准确性。肾病组的尿沉渣和上清液中检测到许多CE种类，而另一组中仅检测到少数CE种类。在肾病组中，所有CE种类的浓度总和在尿样的沉渣和上清液之间显示出显著相关性（r = 0.876，p < 0.001）；然而，它们之间CE的组成有显著差异。此外，上清液中CE的组成与血清相似。
我们的LC-MS/MS分析揭示了肾病患者尿沉渣中独特的CE谱，表明尿路上皮细胞中的CE可能对肾病的发展有贡献。
