Yokoyama S, Niimi S, Tsuruoka M, Kijima T, Fukuda Y, Komuro N, Terashima Y
Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo.
Nihon Sanka Fujinka Gakkai Zasshi. 1988 Dec;40(12):1867-74.
Recently it has been suggested that proto-oncogene plays a role not only in cellular proliferation, development and differentiation, but also in neoplastic transformation. We now show the expression and its localization of c-myc, c-fms and c-sis proto-oncogenes in human developing chorionic tissue and fresh surgical specimens of mole and choriocarcinoma with the method of Northern blotting and In-situ hybridization. The 2.4kb c-myc transcript has been localized to the cytotrophoblast in early placenta and also localized to the C and S typed trophoblastic cells in mole and choriocarcinoma. The 4.0kb c-fms transcript has been localized to the syncytiotrophoblast, especially the highly differentiated syncytiotrophoblast in the second and third trimesters and S typed trophoblastic cells in mole and choriocarcinoma. Moreover, the 4.0kb c-sis transcript has been localized to the cytotrophoblast in early placenta, but not detected in mole or choriocarcinoma. First, these results suggest that the stage and site specific expression of c-myc, c-fms and c-sis proto-oncogenes are clearly related to the proliferation, development and differentiation of normal trophoblastic cells. Second, the expression of c-myc, c-fms proto-oncogenes may be of particular importance in the tumorigenesis and progression of trophoblastic disease.
最近有人提出，原癌基因不仅在细胞增殖、发育和分化中起作用，而且在肿瘤转化中也起作用。我们现在用Northern印迹法和原位杂交法显示了c-myc、c-fms和c-sis原癌基因在人发育中的绒毛膜组织以及葡萄胎和绒毛膜癌的新鲜手术标本中的表达及其定位。2.4kb的c-myc转录本在早期胎盘的细胞滋养层中定位，在葡萄胎和绒毛膜癌的C型和S型滋养层细胞中也定位。4.0kb的c-fms转录本定位在合体滋养层，特别是在妊娠中期和晚期高度分化的合体滋养层以及葡萄胎和绒毛膜癌的S型滋养层细胞中。此外，4.0kb的c-sis转录本在早期胎盘的细胞滋养层中定位，但在葡萄胎或绒毛膜癌中未检测到。首先，这些结果表明c-myc、c-fms和c-sis原癌基因的阶段和位点特异性表达与正常滋养层细胞的增殖、发育和分化明显相关。其次，c-myc、c-fms原癌基因的表达可能在滋养层疾病的肿瘤发生和进展中特别重要。
