da Silva Aleksandro S, Baldissera Matheus D, Bottari Nathieli B, Doleski Pedro H, Carmo Guilherme M, Schwertz Claiton I, Lucca Neuber J, Henker Luan C, Piva Manoela M, Giacomin Patrícia, Stefani Lenita M, Leal Daniela B R, Machado Gustavo, Mendes Ricardo E
Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, Santa Catarina, SC, Brazil; Graduate Program in Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
Comp Immunol Microbiol Infect Dis. 2017 Apr;51:9-13. doi: 10.1016/j.cimid.2017.01.001. Epub 2017 Feb 7.
The aim of this study was to evaluate the influence of dictyocaulosis (mild or severe) on enzymes of NTPDase, 5'-nucleotidase, and adenosine deaminase (ADA) of dairy cows naturally infected by Dictyocaulus viviparus. Blood and faeces were collected from 22 dairy cows of the same farm to evaluate NTPDase (ATP and ADP substrate), 5'-nucleotidase, and ADA activities on days 0 (pre-treatment) and 10 (post-treatment). Seric activities of NTPDase (ATP substrate), 5'-nucleotidase, and ADA were lower (P<0.05) in D. viviparus infected animals compared to uninfected cows. The number of D. viviparus larvae per gram of faeces varied among the animals, and they showed different degrees of severity according to respiratory clinical signs of the disease (cough and nasal discharge). Later, these cows were divided into two groups: those with mild (n=10) and severe (n=12) disease. Cows with severe disease showed higher NTPDase activity (ATP substrate) than those with mild disease (P≤0.05). The opposite occurred with NTPDase (ADP substrate), 5'-nucleotidase, and ADA in cows with severe disease, that is, the enzymatic activity of these seric enzymes significantly decreased (P≤0.05) compared to animals with mild disease. Infected animals showed reduced NTPDase activity (ATP and ADP substrate) after treatment. No enzymatic changes were observed for 5'-nucleotidase, and ADA pre- and post-treatment (P>0.05). Based on these results, we conclude that dictyocaulosis alters NTPDase, 5'-nucleotidase, and ADA activities of cow naturally infected by the parasite, in consequence the enzymes act as inflammatory markers.
本研究的目的是评估网尾线虫病（轻度或重度）对自然感染胎生网尾线虫的奶牛的NTPD酶、5'-核苷酸酶和腺苷脱氨酶（ADA）的影响。从同一农场的22头奶牛采集血液和粪便，以评估在第0天（治疗前）和第10天（治疗后）的NTPD酶（ATP和ADP底物）、5'-核苷酸酶和ADA活性。与未感染的奶牛相比，感染胎生网尾线虫的动物血清中NTPD酶（ATP底物）、5'-核苷酸酶和ADA的活性较低（P<0.05）。每克粪便中胎生网尾线虫幼虫的数量在动物之间有所不同，并且根据疾病的呼吸道临床症状（咳嗽和鼻分泌物）表现出不同程度的严重程度。随后，这些奶牛被分为两组：轻度疾病组（n = 10）和重度疾病组（n = 12）。患有严重疾病的奶牛显示出比患有轻度疾病的奶牛更高的NTPD酶活性（ATP底物）（P≤0.05）。在患有严重疾病的奶牛中，NTPD酶（ADP底物）、5'-核苷酸酶和ADA的情况则相反，即与患有轻度疾病的动物相比，这些血清酶的酶活性显著降低（P≤0.05）。感染动物在治疗后显示出NTPD酶活性（ATP和ADP底物）降低。5'-核苷酸酶和ADA在治疗前后未观察到酶活性变化（P>0.05）。基于这些结果，我们得出结论，网尾线虫病会改变被该寄生虫自然感染的奶牛的NTPD酶、5'-核苷酸酶和ADA活性，因此这些酶可作为炎症标志物。
