橄榄树修剪生物质产生的低聚糖对双歧杆菌的生长刺激作用。

Bifidobacterial growth stimulation by oligosaccharides generated from olive tree pruning biomass.

机构信息

Department of Chemical, Environmental and Materials Engineering, Universidad de Jaén, Campus Las Lagunillas, 23071, Jaén, Spain.

Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.

出版信息

Carbohydr Polym. 2017 Aug 1;169:149-156. doi: 10.1016/j.carbpol.2017.04.014. Epub 2017 Apr 9.

DOI:10.1016/j.carbpol.2017.04.014

PMID:28504130

Abstract

This work aims to evaluate the prebiotic potential of oligosaccharides (OS) obtained from autohydrolysis of olive tree pruning biomass (OTPB). Two selected fractions (F1 and F2) were characterized and used in in vitro fermentations by two Bifidobacterium spp. (B. adolescentis and B. longum) and one fecal inoculum. The fraction F1 presented a lower average degree of polymerization (DP) mainly with OS ranging from 3 to 6 DP, whereas the fraction F2 corresponded to a pool of unsubstituted and acetylated oligomers with DP between 4 and 19. In the fermentation by Bifidobacterium, F1 supported a higher biomass formation, OS consumption and organic acids production than F2. With the fecal inoculum, the accumulation of organic acids, as the sum of acetate, propionate and butyrate, was similar for F1 and F2 (107 and 101mM, respectively). The bifidobacteria counts also increased during the incubation time for both OS fractions.

摘要

本研究旨在评估橄榄树修剪生物质（OTPB）自水解得到的低聚糖（OS）的益生元潜力。对两种选定的低聚糖（F1 和 F2）进行了表征，并使用两种双歧杆菌（B. adolescentis 和 B. longum）和一个粪便接种物进行体外发酵。F1 具有较低的平均聚合度（DP），主要含有 3 到 6 DP 的 OS，而 F2 则是未取代和乙酰化低聚物的混合物，DP 范围为 4 到 19。在双歧杆菌发酵中，F1 支持更高的生物量形成、OS 消耗和有机酸生成，优于 F2。对于粪便接种物，F1 和 F2 的有机酸积累量（乙酸、丙酸和丁酸的总和）相似（分别为 107 和 101mM）。在两种 OS 片段的孵育过程中，双歧杆菌的数量也增加了。