Department of Applied Chemistry and Microbiology, FIN-00014 University of Helsinki, Finland.
J Agric Food Chem. 2009 Sep 23;57(18):8598-606. doi: 10.1021/jf901397b.
Bifidobacterium adolescentis ATCC 15703, Bifidobacterium breve ATCC 15700, Bifidobacterium longum ATCC 15707, and human fecal microbiota were cultivated in vitro with d-xylose, l-arabinose, xylo-oligosaccharides (XOS), and arabinoxylo-oligosaccharides (AXOS) as carbon sources. The pH, formation of volatile fatty acids, and carbohydrate utilization profiles were followed. In the pure bifidobacteria cultures optical density and in the fecal slurries pressure and H(2) were also detected. A differing substrate preference was observed among the various bifidobacteria strains. B. adolescentis grew on XOS, slowly on d-xylose, but not on l-arabinose. In contrast, B. longum preferred l-arabinose and did not grow on pure d-xylose or XOS. Both strains were able to utilize AXOS but with differing strategies, since after the cleavage of l-arabinose B. adolescentis consumed the XOS formed, whereas B. longum fermented the l-arabinose released. B. breve grew poorly on all of the substrates provided. A bifidobacterial mixture and the fecal microbiota were able to utilize pure singly substituted AXOS almost completely, but pure AXOS with a doubly substituted xylose residue was fermented only by the fecal microbiota. Thus, AXOS appear to be potential candidates for slowly fermenting prebiotics, but their prebiotic effects may be dependent on the type of arabinose substitution and the presence of other carbohydrates.
青春双歧杆菌 ATCC 15703、短双歧杆菌 ATCC 15700、长双歧杆菌 ATCC 15707 和人体粪便微生物群在体外分别以 d-木糖、l-阿拉伯糖、木二糖（XOS）和阿拉伯木聚糖寡糖（AXOS）为碳源进行培养。跟踪 pH 值、挥发性脂肪酸的形成和碳水化合物的利用情况。在纯双歧杆菌培养物中检测到光密度，在粪便悬浮液中检测到压力和 H2。观察到各种双歧杆菌菌株之间存在不同的底物偏好。B. adolescentis 可在 XOS 上生长，在 d-木糖上缓慢生长，但不能在 l-阿拉伯糖上生长。相比之下，B. longum 更喜欢 l-阿拉伯糖，并且不能在纯 d-木糖或 XOS 上生长。两种菌株都能够利用 AXOS，但策略不同，因为在 l-阿拉伯糖裂解后，B. adolescentis 消耗形成的 XOS，而 B. longum 发酵释放的 l-阿拉伯糖。B. breve 在所有提供的底物上生长都很差。双歧杆菌混合物和粪便微生物群几乎可以完全利用纯单取代的 AXOS，但含有双取代木糖残基的纯 AXOS 仅被粪便微生物群发酵。因此，AXOS 似乎是潜在的缓慢发酵的益生元候选物，但它们的益生元作用可能取决于阿拉伯糖取代的类型和其他碳水化合物的存在。
