四氧嘧啶自由基从铁蛋白中释放铁。

Iron release from ferritin by alloxan radical.

作者信息

Miura T, Sakurai K

机构信息

Department of Biochemistry, Hokkaido Institute of Pharmaceutical Sciences, Otaru, Japan.

出版信息

Life Sci. 1988;43(25):2145-9. doi: 10.1016/0024-3205(88)90365-7.

DOI:10.1016/0024-3205(88)90365-7

PMID:2850425

Abstract

Alloxan in the presence of reduced glutathione released iron from ferritin which is the major intracellular iron storage protein. Superoxide dismutase inhibited by only about 30% the alloxan-dependent iron release from ferritin but completely inhibited the iron release from ferritin induced by hypoxanthine-xanthine oxidase. Under anaerobic conditions, the ESR spectrum of alloxan radical was obtained and interaction with ferritin resulted in a marked diminution of the alloxan radical signal. These results indicate that alloxan radical rapidly releases iron from ferritin.

摘要

在还原型谷胱甘肽存在的情况下，四氧嘧啶从作为主要细胞内铁储存蛋白的铁蛋白中释放铁。超氧化物歧化酶仅抑制约30%的四氧嘧啶依赖性铁从铁蛋白的释放，但完全抑制次黄嘌呤 - 黄嘌呤氧化酶诱导的铁从铁蛋白的释放。在厌氧条件下，获得了四氧嘧啶自由基的电子顺磁共振谱，并且与铁蛋白的相互作用导致四氧嘧啶自由基信号明显减弱。这些结果表明四氧嘧啶自由基迅速从铁蛋白中释放铁。