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Mexneurin是啮齿动物中枢神经系统中一种新型的肽前体。

Mexneurin is a novel precursor of peptides in the central nervous system of rodents.

作者信息

Matus-Ortega Maura E, Leff Gelman Philippe, Calva-Nieves Juan C, Flores-Zamora Anabel, Salazar-Juárez Alberto, Torner-Aguilar Carlos-Alejandro, Gamba Gerardo, De Los Heros Paola, Peng Bonnie, Pintar John E, Gompf Heinrich S, Allen Charles N, Antón-Palma Benito

机构信息

Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Ciudad de México, México.

Instituto Nacional de Perinatología, Ciudad de México, México.

出版信息

FEBS Lett. 2017 Jun;591(12):1627-1636. doi: 10.1002/1873-3468.12679. Epub 2017 Jun 1.

DOI:10.1002/1873-3468.12679
PMID:28504339
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5509056/
Abstract

Endomorphins (EMs) have been proposed as the endogenous ligand agonists of the μ-opioid receptor; however, no propeptide precursor protein for EMs has been identified. Here, to identify the presumed precursor of EMs, we designed an immunoscreening assay using specific affinity-purified rabbit antisera raised against synthetic EMs in a whole-mouse brain cDNA library. Following this approach, we identify a DNA sequence encoding a protein precursor, which we name proMexneurin, that contains three different peptide sequences: Mexneurin-1 (an EM-like peptide), Mexneurin-2, and Mexneurin-3, a peptide which appears to be unrelated to EMs. RT-PCR analysis and in situ hybridization reveal a widespread distribution of proMexneurin mRNA throughout the mouse brain. Both Mexneurin-1 and Mexneurin-3 peptides display biological activities in the mouse CNS.

摘要

内吗啡肽（EMs）被认为是μ-阿片受体的内源性配体激动剂；然而，尚未鉴定出EMs的前体肽前体蛋白。在此，为了鉴定假定的EMs前体，我们设计了一种免疫筛选试验，使用在全小鼠脑cDNA文库中针对合成EMs产生的特异性亲和纯化兔抗血清。按照这种方法，我们鉴定出一个编码蛋白质前体的DNA序列，我们将其命名为proMexneurin，它包含三种不同的肽序列：Mexneurin-1（一种类EM肽）、Mexneurin-2和Mexneurin-3，一种似乎与EMs无关的肽。RT-PCR分析和原位杂交显示proMexneurin mRNA在整个小鼠脑中广泛分布。Mexneurin-1和Mexneurin-3肽在小鼠中枢神经系统中均表现出生物学活性。

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本文引用的文献

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The biology and therapeutic targeting of the proprotein convertases.蛋白转化酶的生物学与治疗靶向。
Nat Rev Drug Discov. 2012 May;11(5):367-83. doi: 10.1038/nrd3699.
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The proprotein convertases, 20 years later.20年后的前体蛋白转化酶。
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Endomorphin 1 and endomorphin 2 suppress in vitro antibody formation at ultra-low concentrations: anti-peptide antibodies but not opioid antagonists block the activity.内吗啡肽1和内吗啡肽2在超低浓度下可抑制体外抗体形成：抗肽抗体而非阿片类拮抗剂可阻断该活性。
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Search of the human proteome for endomorphin-1 and endomorphin-2 precursor proteins.在人类蛋白质组中搜索内吗啡肽-1和内吗啡肽-2前体蛋白。
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Colocalization and shared distribution of endomorphins with substance P, calcitonin gene-related peptide, gamma-aminobutyric acid, and the mu opioid receptor.内吗啡肽与P物质、降钙素基因相关肽、γ-氨基丁酸及μ阿片受体的共定位和共同分布。
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Chemokines and chemokine receptors in the brain: implication in neuroendocrine regulation.大脑中的趋化因子与趋化因子受体：对神经内分泌调节的影响
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