Lytle C D, Carney P G, Lee W, Bushar H F
Office of Science and Technology, Center for Devices and Radiological Health, FDA, Rockville, MD 20857.
Mutagenesis. 1988 Jul;3(4):297-8. doi: 10.1093/mutage/3.4.297.
The existence of untargeted viral mutagenesis in X-irradiated cells was investigated in a mammalian virus/cell system, where a low level of such viral mutagenesis can be demonstrated in UV-irradiated cells. In the positive control experiment UV-elicited mutagenesis was shown with cell exposures of 5, 10 and 15 J/m2 and a delay of 24 h between cell irradiation and infection with unirradiated herpes simplex virus. Although X-ray doses of 1, 3 and 10 Gy elicit enhanced reactivation of UV-irradiated virus, no untargeted mutagenesis for any X-ray dose at post-irradiation infection times of 0, 24 or 72 h was observed in this study. Thus untargeted mutagenesis of herpes simplex virus was not demonstrated in X-irradiated monkey cells, under conditions where X-ray-enhanced reactivation occurs and where untargeted mutagenesis in UV-irradiated cells occurs.
在一个哺乳动物病毒/细胞系统中研究了X射线照射细胞中存在的非靶向病毒诱变，在该系统中，紫外线照射的细胞中可以证明存在低水平的这种病毒诱变。在阳性对照实验中，细胞暴露于5、10和15 J/m²的紫外线，并在细胞照射和未照射的单纯疱疹病毒感染之间延迟24小时，显示出紫外线诱导的诱变。尽管1、3和10 Gy的X射线剂量可引发紫外线照射病毒的增强再活化，但在本研究中，在照射后0、24或72小时的感染时间，未观察到任何X射线剂量的非靶向诱变。因此，在发生X射线增强再活化以及紫外线照射细胞中发生非靶向诱变的条件下，未在X射线照射的猴细胞中证明单纯疱疹病毒的非靶向诱变。
