Garvin A M, Pawar S, Marth J D, Perlmutter R M
Howard Hughes Medical Institute, University of Washington, Seattle 98195.
Mol Cell Biol. 1988 Aug;8(8):3058-64. doi: 10.1128/mcb.8.8.3058-3064.1988.
The lck gene encodes a lymphocyte-specific protein-tyrosine kinase that is implicated in neoplastic transformation. We have determined the germ line organization of the murine lck gene and have isolated and characterized a rearranged lck allele in the murine lymphoma cell line LSTRA. The overall exon-intron organization of the normal lck gene is almost identical to that of avian c-src. In LSTRA DNA, an internally rearranged Moloney murine leukemia virus genome is interposed between two distinct promoters that normally generate lck transcripts differing only in 5' untranslated regions. The rearrangement appears to have been selected to permit splicing of transcripts that initiate from the Moloney virus promoter to an acceptor site located within the first exon 3' to the downstream promoter, thus generating an lck mRNA with a novel 5' untranslated region that may be more efficiently translated.
lck基因编码一种与肿瘤转化有关的淋巴细胞特异性蛋白酪氨酸激酶。我们已经确定了小鼠lck基因的种系结构，并在小鼠淋巴瘤细胞系LSTRA中分离和鉴定了一个重排的lck等位基因。正常lck基因的外显子-内含子总体结构与禽c-src几乎相同。在LSTRA DNA中，一个内部重排的莫洛尼鼠白血病病毒基因组插入到两个不同的启动子之间，这两个启动子通常产生仅在5'非翻译区不同的lck转录本。这种重排似乎是为了允许从莫洛尼病毒启动子起始的转录本剪接到位于下游启动子3'端第一个外显子内的一个受体位点，从而产生一种具有可能更高效翻译的新5'非翻译区的lck mRNA。
