Sehgal A, Patil N, Chao M
Department of Cell Biology and Anatomy, Cornell University Medical College, New York, New York 10021.
Mol Cell Biol. 1988 Aug;8(8):3160-7. doi: 10.1128/mcb.8.8.3160-3167.1988.
Expression of nerve growth factor receptor is normally restricted to cells derived from the neural crest in a developmentally regulated manner. We analyzed promoter sequences for the human nerve growth factor receptor gene and found that the receptor promoter resembles others which are associated with constitutively expressed genes that have housekeeping and growth-related functions. Unlike these other genes, the initiation of transcription occurred at one major site rather than at multiple sites. The constitutive nature of the nerve growth factor receptor promoter may account for the ability of this gene to be transcribed in a diverse number of heterologous cells after gene transfer. The intron-exon structure of the receptor gene indicated that structural features are precisely divided into discrete domains.
神经生长因子受体的表达通常以发育调控的方式局限于源自神经嵴的细胞。我们分析了人类神经生长因子受体基因的启动子序列，发现该受体启动子与其他一些启动子相似，这些启动子与具有管家和生长相关功能的组成型表达基因相关。与这些其他基因不同，转录起始发生在一个主要位点而非多个位点。神经生长因子受体启动子的组成型性质可能解释了该基因在基因转移后能够在多种异源细胞中被转录的能力。受体基因的内含子-外显子结构表明，结构特征被精确地划分为离散的结构域。
