Chen Xinhua, Ren Zhigang, Yin Shengyong, Xu Yuning, Guo Danjing, Xie Haiyang, Zhou Lin, Wu Liming, Jiang Jianwen, Li Hongchun, Sun Junhui, Zheng Shusen
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University, Hangzhou 310003, China.
Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Cytokine. 2017 May;93:44-50. doi: 10.1016/j.cyto.2017.05.003. Epub 2017 May 12.
How irreversible electroporation (IRE) affect immune status is still kept unknown. This preclinical study is to investigate its local and systemic immune reaction both on tumor-bearing and tumor free animals.
Liver ablation was performed by a standard IRE instrument and proposal. Altogether 57 tumor bearing mice and 10 tumor-free porcine livers were ablated. The reaction of survival, radiology image, pathologically and immunologically were followed up. The detailed cytokines and chemokines responses were recorded dynamically post IRE ablation.
IRE ablation induced coagulation and necrosis in liver. It caused macrophages infiltration, in ablation zone. IRE ablation caused cellular inflammation. It, corrected the abnormal drifted Th2 in HCC back to Th1 status, promoting tumor eradication and host survival. The quantified cytokines and chemokines indicate IRE can stimulate both local immune reaction and systemic immune reaction.
Local IRE ablation changes the abnormal drifted Th2 in HCC back to Th1 status, facilitating tumor eradication and host survival.
不可逆电穿孔（IRE）如何影响免疫状态仍不清楚。这项临床前研究旨在调查其在荷瘤动物和无瘤动物身上的局部和全身免疫反应。
使用标准IRE仪器和方案进行肝脏消融。总共对57只荷瘤小鼠和10个无瘤猪肝进行了消融。对生存、放射学图像、病理和免疫反应进行随访。在IRE消融后动态记录详细的细胞因子和趋化因子反应。
IRE消融导致肝脏发生凝固性坏死。它导致巨噬细胞浸润到消融区域。IRE消融引起细胞炎症。它将肝癌中异常漂移的Th2校正回Th1状态，促进肿瘤根除和宿主存活。定量的细胞因子和趋化因子表明IRE可刺激局部免疫反应和全身免疫反应。
局部IRE消融将肝癌中异常漂移的Th2校正回Th1状态，促进肿瘤根除和宿主存活。
