Production of active human carbonic anhydrase II in E. coli.
作者信息
Forsman C, Behravan G, Osterman A, Jonsson B H
机构信息
Department of Biochemistry, University of Umeå, Sweden.
出版信息
Acta Chem Scand B. 1988 May;42(5):314-8. doi: 10.3891/acta.chem.scand.42b-0314.
Abstract
cDNA encoding human carbonic anhydrase II has been isolated and its nucleotide sequence determined. Expression of the isolated carbonic anhydrase gene in Escherichia coli from a plasmid containing the tac promoter yielded an active enzyme at a level of about 1% of total protein.
摘要
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