Putri Dwi Desmiyeni, Handharyani Ekowati, Soejoedono Retno Damajanti, Setiyono Agus, Mayasari Ni Luh Putu Ika, Poetri Okti Nadia
Study Program of Animal Biomedical Science, IPB Graduate School, Bogor Agricultural University, Bogor, Indonesia.
Study Program of Animal Husbandry, Department of Animal Husbandry, State Polytechnic of Lampung, Lampung, Indonesia.
Vet World. 2017 Apr;10(4):438-444. doi: 10.14202/vetworld.2017.438-444. Epub 2017 Apr 22.
This research was conducted to differentiate and characterize eight Newcastle disease virus (NDV) isolates collected from vaccinated chicken at commercial flocks in West Java, Indonesia, in 2011, 2014 and 2015 by pathotype specific primers.
A total of eight NDV isolates collected from clinical outbreaks among commercial vaccinated flocks in West Java, Indonesia, in 2011, 2014, and 2015 were used in this study. Reverse transcription-polymerase chain reaction was used to detect and differentiate virulence of NDV strains, using three sets of primers targeting their M and F gene. First primers were universal primers to detect NDV targeting matrix (M) gene. Other two sets of primers were specific for the fusion (F) gene cleavage site sequence of virulent and avirulent NDV strains.
Our results showed that three isolates belong to NDV virulent strains, and other five isolates belong to NDV avirulent strains. The nucleotide sequence of the F protein cleavage site showed K/R-R-Q/R-K-R/G-F on NDV virulent strains and G-K/R-Q-G-R-L on NDV avirulent strain.
Result from the current study suggested that NDV virulent strain were circulating among vaccinated chickens in West Java, Indonesia; this might possess a risk of causing ND outbreaks and causing economic losses within the poultry industry.
本研究旨在通过致病型特异性引物对2011年、2014年和2015年从印度尼西亚西爪哇商业鸡群中接种疫苗的鸡身上分离得到的8株新城疫病毒（NDV）进行鉴别和特征分析。
本研究使用了2011年、2014年和2015年从印度尼西亚西爪哇商业接种鸡群临床暴发中分离得到的8株新城疫病毒。采用逆转录聚合酶链反应，使用针对其M和F基因的三组引物检测和区分新城疫病毒株的毒力。第一组引物是检测新城疫病毒靶向基质（M）基因的通用引物。另外两组引物分别针对强毒和无毒新城疫病毒株的融合（F）基因裂解位点序列。
我们的结果表明，3株分离株属于新城疫病毒强毒株，另外5株分离株属于新城疫病毒无毒株。F蛋白裂解位点的核苷酸序列在新城疫病毒强毒株上显示为K/R-R-Q/R-K-R/G-F, 在新城疫病毒无毒株上显示为G-K/R-Q-G-R-L。
本研究结果表明，新城疫病毒强毒株在印度尼西亚西爪哇接种疫苗的鸡群中传播；这可能存在引发新城疫暴发并给家禽业造成经济损失的风险。
