Abdel-Hady Ahmed, El-Hindawi Ali, Hammam Olfat, Khalil Heba, Diab Sara, El-Aziz Soulafa Abd, Badawy Mohamed, Ismail Ahmed, Helmy Noha, Kamel Nora, Anis Shady, Kholy Amr El, Osili Khalid Al, Abdel-Hady Afaf, Nour Hani, Akl Maha
Theodor Bilharz Research Institute, Imbaba, Giza, Cairo, Egypt.
Faculty of Medicine Cairo University, Cairo, Egypt.
Open Access Maced J Med Sci. 2017 Mar 20;5(2):147-154. doi: 10.3889/oamjms.2017.037. eCollection 2017 Apr 15.
Prostate cancer (PCa) is the second most common cancers in men worldwide. Its incidence can be influenced by several risk factors including genetic susceptibility. Therefore the search for the expression of a certain gene (ERG) and its rearrangement could give us clues for proper identification of PCa. And the study of ERG expression and its comparison to FISH in Egyptian patients can show whether ERG immunophenotype could be used instead of FISH, as it is cheaper.
This study was performed on 85 cases of PCa, showing 30 cases with HGPIN and 30 cases of prostatic hyperplasia. All were immunohistochemistry stained using ERG monoclonal rabbit antihuman antibody was used (clone: EP111). FISH analysis was performed in 38 biopsies of PCa cases to detect TMRPSS2-ERG rearrangement using the FISH ZytoLight TriCheck Probe (SPEC TMRPSS2-ERG).
ERG expression was found in 26% of PCa cases and 20% of HGPIN cases. FISH analysis showed fusion of 21 cases of PCa (out of 22 cases showing ERG immunoexpression).
Our findings emphasise that only malignant and pre-malignant cells and not benign cells from the prostate stain positive. ERG expression may offer a simpler, accurate and less costly alternative for evaluation of ERG fusion status in PCa.
前列腺癌（PCa）是全球男性中第二常见的癌症。其发病率会受到包括遗传易感性在内的多种风险因素的影响。因此，寻找特定基因（ERG）的表达及其重排可为我们准确识别前列腺癌提供线索。并且对埃及患者的ERG表达及其与荧光原位杂交（FISH）的比较研究可以表明ERG免疫表型是否可因其成本较低而替代FISH使用。
本研究对85例前列腺癌病例进行了分析，其中30例为高级别前列腺上皮内瘤变（HGPIN），30例为前列腺增生。所有病例均使用ERG单克隆兔抗人抗体（克隆号：EP111）进行免疫组织化学染色。对38例前列腺癌活检病例进行FISH分析，使用FISH ZytoLight TriCheck探针（SPEC TMRPSS2-ERG）检测TMRPSS2-ERG重排。
在26%的前列腺癌病例和20%的高级别前列腺上皮内瘤变病例中发现了ERG表达。FISH分析显示22例显示ERG免疫表达的前列腺癌病例中有21例发生融合。
我们的研究结果强调，只有前列腺的恶性和癌前细胞而非良性细胞染色呈阳性。ERG表达可能为评估前列腺癌中ERG融合状态提供一种更简单、准确且成本更低的替代方法。
