Venâncio Paulo César, Raimundo Figueroba Sidney, Dias Nani Bruno, Eduardo Nunes Ferreira Luiz, Vilela Muniz Bruno, de Sá Del Fiol Fernando, Sartoratto Adilson, Antonio Ribeiro Rosa Edvaldo, Carlos Groppo Francisco
Department of Exact Sciences, Technical School of Limeira, Cotil, UNICAMP, Limeira, São Paulo, Brazil.
Department of Physiological Sciences, Piracicaba Dental School, UNICAMP, Piracicaba, São Paulo, Brazil.
Adv Pharm Bull. 2017 Apr;7(1):115-121. doi: 10.15171/apb.2017.015. Epub 2017 Apr 13.
This study observed the effect of garlic extracts and amoxicillin against an induced staphylococcal infection model. MIC and MBC were also obtained for aqueous extracts of Allium sativum (Asa) and Allium tuberosum (Atu) against Staphylococcus aureus penicillin-sensitive (PSSA - ATCC 25923) and MRSA (ATCC 33592). Granulation tissues were induced in the back of 205 rats. After 14 days, 0.5 mL of 10 CFU/mL of PSSA or MRSA were injected inside tissues. After 24h, animals were divided: G1 (Control) - 0.5 mL of NaCl 0.9%; G2 - Asa 100 mg/kg or 400mg/kg; G3 - Atu 100 mg/kg or 400 mg/kg; G4 - amoxicillin suspension 50 mg/kg, considering PSSA infection; and G5 (Control) - 0.5 mL of NaCl 0.9%; G6 - Asa 400mg/kg; G7 - amoxicillin 50 mg/kg; and G8 - Asa 400 mg/kg + amoxicillin 50 mg/kg for MRSA. All treatments were administered P.O. every 6h. Animals were killed at 0, 6, 12 and 24h. Samples were spread on salt-mannitol agar. Colonies were counted after 18 h at 37 °C. Atu was not able to inhibit or kill PSSA and MRSA. Considering Asa, MIC and MBC against PSSA were 2 mg/mL and 4 mg/mL, respectively; and 16 mg/mL and 64 mg/mL against MRSA. No effect was observed in vivo for control, Asa 100 mg/kg and Atu 100 mg/kg, while amoxicillin, Atu 400 mg/kg and Asa 400 mg/kg decreased PSSA counts in all-time points. No effect of any group against MRSA was observed at any time. Thus, A. sativum and A. tuberosum were able to reduce PSSA infection, but not MRSA infection.
本研究观察了大蒜提取物和阿莫西林对诱导性葡萄球菌感染模型的作用。还测定了大蒜（Asa）和韭菜（Atu）水提取物对青霉素敏感金黄色葡萄球菌（PSSA - ATCC 25923）和耐甲氧西林金黄色葡萄球菌（MRSA - ATCC 33592）的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。在205只大鼠背部诱导形成肉芽组织。14天后，将0.5 mL含10 CFU/mL的PSSA或MRSA注射到组织内。24小时后，将动物分组：G1（对照组） - 0.5 mL 0.9%氯化钠溶液；G2 - Asa 100 mg/kg或400mg/kg；G3 - Atu 100 mg/kg或400 mg/kg；G4 - 阿莫西林混悬液50 mg/kg（针对PSSA感染）；G5（对照组） - 0.5 mL 0.9%氯化钠溶液；G6 - Asa 400mg/kg；G7 - 阿莫西林50 mg/kg；G8 - Asa 400 mg/kg + 阿莫西林50 mg/kg（针对MRSA）。所有治疗均口服给药，每6小时一次。在0、6、12和24小时处死动物。将样本接种于盐甘露醇琼脂平板上。在37℃培养18小时后计数菌落。Atu无法抑制或杀灭PSSA和MRSA。对于Asa，其对PSSA的MIC和MBC分别为2 mg/mL和4 mg/mL；对MRSA的MIC和MBC分别为16 mg/mL和64 mg/mL。对照组、Asa 100 mg/kg组和Atu 100 mg/kg组在体内未观察到效果，而阿莫西林、Atu 400 mg/kg组和Asa 400 mg/kg组在所有时间点均降低了PSSA的数量。在任何时间，任何组对MRSA均未观察到效果。因此，大蒜和韭菜能够减轻PSSA感染，但不能减轻MRSA感染。
