MDM2基因启动子T309G多态性与子痫前期易感性相关。
The MDM2 promoter T309G polymorphism was associated with preeclampsia susceptibility.
作者信息
Salimi Saeedeh, Mohammadpour-Gharehbagh Abbas, Rezaei Mahnaz, Sajadian Mojtaba, Teimoori Batool, Yazdi Atefeh, Mokhtari Mojgan, Yaghmaei Minoo
机构信息
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
Department of Clinical Biochemistry, School of Medicine and Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
出版信息
J Assist Reprod Genet. 2017 Jul;34(7):951-956. doi: 10.1007/s10815-017-0941-3. Epub 2017 May 15.
PURPOSE
Preeclampsia (PE) is a hypertensive disorder of pregnancy in which abnormal proliferation and apoptosis of placenta trophoblast has a pivotal role in its pathophysiology. The aim of the current study was to examine the association between Mouse Double Minute 2 (MDM2) T309G and 40 bp insertion/deletion (I/D) polymorphisms and PE risk.
METHODS
A case-control study was conducted on 208 PE women and 164 healthy pregnant women matching age, sex, and ethnicity. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PCR methods were used for genotyping.
RESULTS
The MDM2 309GG genotype was associated with PE, and this genotype was found to be a risk factor for PE. There was no association between the MDM2 I/D polymorphism and PE. The haplotype-based association analysis revealed no association between MDM2 T309G and 40 bp I/D polymorphisms and PE. The frequency of TT-DD and GG-DD combined genotypes were significantly higher in PE women with marginal P values (P = 0.046).
CONCLUSIONS
The MDM2 309GG genotype was associated with higher risk of PE. The TT-DD and GG-DD combined genotypes were higher in PE women.
目的
子痫前期（PE）是一种妊娠期高血压疾病，胎盘滋养层细胞的异常增殖和凋亡在其病理生理学中起关键作用。本研究旨在探讨小鼠双微体2（MDM2）基因T309G和40bp插入/缺失（I/D）多态性与PE风险之间的关联。
方法
对208例PE患者和164例年龄、性别和种族匹配的健康孕妇进行病例对照研究。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和PCR方法进行基因分型。
结果
MDM2 309GG基因型与PE相关，且该基因型是PE的一个危险因素。MDM2 I/D多态性与PE之间无关联。基于单倍型的关联分析显示，MDM2 T309G和40bp I/D多态性与PE之间无关联。PE患者中TT-DD和GG-DD联合基因型的频率显著更高，P值接近临界值（P = 0.046）。
结论
MDM2 309GG基因型与PE的高风险相关。PE患者中TT-DD和GG-DD联合基因型的频率更高。
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