在从人血浆样本进行电膜萃取过程中对磷脂的高效鉴别与去除。
Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples.
作者信息
Vårdal Linda, Gjelstad Astrid, Huang Chuixiu, Øiestad Elisabeth Leere, Pedersen-Bjergaard Stig
机构信息
School of Pharmacy, University of Oslo, PO Box 1068, Blindern, 0316 Oslo, Norway.
Department of Forensic Sciences, Oslo University Hospital, PO Box 4950, Nydalen, 0424 Oslo, Norway.
出版信息
Bioanalysis. 2017 Apr;9(8):631-641. doi: 10.4155/bio-2017-0027. Epub 2017 May 16.
AIM
For the first time, extracts obtained from human plasma samples by electromembrane extraction (EME) were investigated comprehensively with particular respect to phospholipids using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Thhe purpose was to investigate the potential of EME for phospholipid cleanup in different EME systems.
RESULTS & DISCUSSION: No traces of phospholipids were detected in any of the acceptor solutions, whereas the model analytes were extracted with recoveries up to 50%. Thus, the EME systems tested in this work were found to be highly efficient for providing phospholipid-free extracts.
CONCLUSION
Ultra-HPLC-MS/MS analysis of the donor solutions revealed that the phospholipids principally remained in the plasma samples. This proved that the phospholipids did not migrate in the electrical field and they were prevented from penetrating the supported liquid membrane.
目的
首次使用超高效液相色谱串联质谱法（UHPLC-MS/MS），特别针对磷脂，对通过电膜萃取（EME）从人血浆样本中获得的提取物进行了全面研究。目的是研究EME在不同EME系统中进行磷脂净化的潜力。
结果与讨论
在任何受体溶液中均未检测到磷脂痕迹，而模型分析物的提取回收率高达50%。因此，在这项工作中测试的EME系统被发现对于提供无磷脂提取物非常高效。
结论
对供体溶液的超高效液相色谱-质谱/质谱分析表明，磷脂主要保留在血浆样本中。这证明磷脂不会在电场中迁移，并且它们被阻止穿透支撑液膜。
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