Wagner-Johnston Nina D, Lensing Shelly, Noy Ariela, Ratner Lee, Henry David, Lee Jeannette Y, Silver Sylvia, Faham Malek, Ambinder Richard F
a Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University , Baltimore , MD , USA.
b University of Arkansas for Medical Sciences , Little Rock , AK , USA.
Leuk Lymphoma. 2017 Dec;58(12):2939-2942. doi: 10.1080/10428194.2017.1317095. Epub 2017 May 16.
Patients with HIV are at increased risk for developing B-cell lymphomas likely due in part to chronic antigen stimulation leading to clonal immunoglobulin (Ig) gene rearrangements. Next-generation sequencing (NGS)-based identification of circulating Ig clonotypes has not been well-characterized in HIV-related lymphomas. The AIDS Malignancies Consortium (AMC) enrolled 51 untreated patients with HIV-related B-cell lymphomas and analyzed paired tumor/plasma specimens for Ig clonotypes using an NGS approach (AMC064, NCT00981097). Lymphoma-specific clonotypes (>5% frequency) were identified in 83% (33/40) of tumor specimens. Results from paired tumor/plasma specimens showed identical circulating clonotypes in the plasma from 97% (32/33) of patients. High International Prognostic Index (IPI) scores of 3-4 among patients with B-cell lymphoma correlated with higher lymphoma molecules/million diploid genomes in the plasma compared with lower IPI scores of 0-2, median 77335 vs. 6876, p = .005. Further studies are merited to determine whether plasma clonal Ig DNA is prognostic in HIV-related lymphomas.
感染人类免疫缺陷病毒（HIV）的患者患B细胞淋巴瘤的风险增加，这可能部分归因于慢性抗原刺激导致克隆性免疫球蛋白（Ig）基因重排。基于下一代测序（NGS）的循环Ig克隆型鉴定在HIV相关淋巴瘤中尚未得到充分表征。艾滋病恶性肿瘤协会（AMC）招募了51名未经治疗的HIV相关B细胞淋巴瘤患者，并使用NGS方法（AMC064，NCT00981097）分析了配对的肿瘤/血浆标本中的Ig克隆型。在83%（33/40）的肿瘤标本中鉴定出淋巴瘤特异性克隆型（频率>5%）。配对的肿瘤/血浆标本结果显示，97%（32/33）患者的血浆中存在相同的循环克隆型。B细胞淋巴瘤患者中国际预后指数（IPI）评分为3 - 4与血浆中较高的淋巴瘤分子/百万二倍体基因组相关，而IPI评分为0 - 2时较低，中位数分别为77335和6876，p = 0.005。值得进一步研究以确定血浆克隆性Ig DNA在HIV相关淋巴瘤中是否具有预后价值。
