Yang Qin Qin, Xu Xiao Ping, Zhao Hong Shu, Cai Yue Qin, Pan Yong Ming, Xu Jian Qin, Ma Quan Xin, Chen Min Li
Experimental Animal Research Center, Zhejiang Chinese Medical University, Hangzhou, Zhejiang Province, China.
Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, Zhejiang Province, China.
J Dig Dis. 2017 Jun;18(6):330-342. doi: 10.1111/1751-2980.12485.
This study aimed to evaluate the differential expressions of microRNAs (miRNAs) in white hair black eye (WHBE) rabbits of irritable bowel syndrome (IBS).
WHBE and Japanese white (JW) rabbits were divided into the control and IBS groups. The IBS groups were exposed to moist heat, stress and low-dose laxatives. Their intestinal movement rate was measured. Blood samples were taken to detect serum 5-hydroxytryptamine (5-HT) and dopamine levels and colonic tissues were obtained to detect c-Fos expression by immunohistochemistry. Deep sequencing technology was used to obtain miRNA sequences in the intestinal tissues of WHBE and JW control groups. Expressions of 14 miRNAs were measured by real-time polymerase chain reaction in both the control and the IBS model groups.
Serum 5-HT and dopamine levels, intestinal movement rate and c-Fos expressions in the WHBE rabbits were significantly increased compared with the control group. MiR-29a-3p, miR-24-3p, miR-221-3p, let-7f-5p, let-7g-5p, let-7i-5p, miR-192-5p, miR-126-3p and miR-130b-3p expressions in WHBE IBS rabbits at day 14 were significantly higher than those in the control group while miR-324-3p and miR-132 were downregulated. MiR-29a-3p, let-7i-5p, miR-192-5p and miR-126-3p were significantly upregulated only in JW IBS rabbits at day 14 and miR-324-3p, miR-223-3p and miR-132 were significantly downregulated in JW IBS group. MiR-24-3p, miR-221-3p, let-7f-5p, miR-126-3p and miR-130b-3p expressions in WHBE IBS rabbits were higher than that in JW IBS rabbits.
Twelve miRNAs were differentially expressed in IBS rabbits. Five are specific in WHBE IBS rabbits, suggesting that they play a role in increased sensitivity to IBS.
本研究旨在评估肠易激综合征（IBS）白毛黑眼（WHBE）兔中微小RNA（miRNA）的差异表达。
将WHBE兔和日本白兔（JW）分为对照组和IBS组。IBS组接受湿热、应激和低剂量泻药处理。测量其肠道蠕动率。采集血样检测血清5-羟色胺（5-HT）和多巴胺水平，并获取结肠组织通过免疫组织化学检测c-Fos表达。采用深度测序技术获取WHBE和JW对照组肠道组织中的miRNA序列。通过实时聚合酶链反应在对照组和IBS模型组中检测14种miRNA的表达。
与对照组相比，WHBE兔的血清5-HT和多巴胺水平、肠道蠕动率及c-Fos表达显著升高。在第14天时，WHBE IBS兔中miR-29a-3p、miR-24-3p、miR-221-3p、let-7f-5p、let-7g-5p、let-7i-5p、miR-192-5p、miR-126-3p和miR-130b-3p的表达显著高于对照组，而miR-324-3p和miR-132表达下调。在第14天时，仅JW IBS兔中miR-29a-3p、let-7i-5p、miR-192-5p和miR-126-3p显著上调，JW IBS组中miR-324-3p、miR-223-3p和miR-132显著下调。WHBE IBS兔中miR-24-3p、miR-221-3p、let-7f-5p、miR-126-3p和miR-130b-3p的表达高于JW IBS兔。
12种miRNA在IBS兔中差异表达。其中5种在WHBE IBS兔中具有特异性，提示它们在IBS敏感性增加中发挥作用。
