Reed M J, Gompels M M, Beranek P A, James V H
Department of Chemical Pathology, St. Mary's Hospital Medical School, London, United Kingdom.
Horm Metab Res. 1988 Nov;20(11):713-6. doi: 10.1055/s-2007-1010924.
A direct radioimmunoassay for measuring plasma levels of oestrone sulphate has been developed using 8-anilino-2-naphthalene sulphonic acid to displace oestrone sulphate from plasma binding proteins. Oestrone sulphate was assayed by using an antiserum raised against glucuronide which cross-reacted 100% with oestrone sulphate. The direct assay gave a good analytical recovery of oestrone sulphate and there was a good correlation (r = 0.82, P less than 0.001) for plasma levels of oestrone sulphate measured by the direct assay and a method involving steroid conjugate extraction and enzyme hydrolysis. The mean (+/- S.D.) plasma level of oestrone sulphate in men was 1100 +/- 280 pg/ml. The effect of taking the antibiotic, Ampicillin, on plasma levels of oestrone sulphate was investigated in four men. Plasma levels of oestrone sulphate were significantly reduced after taking Ampicillin for 5 days. Ampicillin may act to lower plasma levels of oestrone sulphate by reducing the growth of bacteria in the gut or by inhibiting oestrogen sulphotransferase activity.
已开发出一种直接放射免疫分析法，用于测量硫酸雌酮的血浆水平，该方法使用8-苯胺基-2-萘磺酸从血浆结合蛋白中置换出硫酸雌酮。通过使用针对葡糖醛酸苷产生的抗血清来测定硫酸雌酮，该抗血清与硫酸雌酮的交叉反应率为100%。直接分析法对硫酸雌酮具有良好的分析回收率，并且通过直接分析法测得的硫酸雌酮血浆水平与一种涉及类固醇缀合物提取和酶水解的方法之间具有良好的相关性（r = 0.82，P < 0.001）。男性硫酸雌酮的平均（±标准差）血浆水平为1100 ± 280 pg/ml。在四名男性中研究了服用抗生素氨苄西林对硫酸雌酮血浆水平的影响。服用氨苄西林5天后，硫酸雌酮的血浆水平显著降低。氨苄西林可能通过减少肠道细菌的生长或抑制雌激素磺基转移酶活性来降低硫酸雌酮的血浆水平。
