Boileau Mélanie J, Mani Rinosh, Clinkenbeard Kenneth D
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma.
Diagnostic Center for Population and Animal Health, College of Veterinary Medicine, Michigan State University, Lansing, Michigan.
Curr Protoc Microbiol. 2017 May 16;45:7B.3.1-7B.3.15. doi: 10.1002/cpmc.29.
Bdellovibrio bacteriovorus 109J is a Gram-negative predatory bacterium with obligate host dependency on other Gram-negative bacteria. This bacteriolytic predator collides with, enters, and establishes growth within the prey (host) periplasm, eventually lysing the prey cell wall to release fresh, motile B. bacteriovorus progeny. Laboratory maintenance of B. bacteriovorus has been previously described by other investigators. The protocols included in this unit deal with the technique required to lyophilize or freeze dry host-dependent B. bacteriovorus. This is an alternative means to frozen glycerol stocks for the long-term storage of B. bacteriovorus. It includes the cultivation process and methods to lyophilize B. bacteriovorus as well as recommended storage conditions. In addition, this unit provides insight on the formulation's shelf-life including the time to active culture after reviving lyophilized stocks of B. bacteriovorus following short-, medium-, and long-term storage. © 2017 by John Wiley & Sons, Inc.
食菌蛭弧菌109J是一种革兰氏阴性捕食细菌，对其他革兰氏阴性细菌具有专性宿主依赖性。这种溶菌性捕食者与猎物（宿主）的周质碰撞、进入并在其中生长，最终裂解猎物细胞壁以释放出新鲜、可运动的食菌蛭弧菌后代。其他研究人员之前已描述过食菌蛭弧菌的实验室保存方法。本单元所包含的方案涉及冻干或冷冻干燥依赖宿主的食菌蛭弧菌所需的技术。这是用于食菌蛭弧菌长期保存的冷冻甘油菌液的替代方法。它包括食菌蛭弧菌的培养过程和冻干方法以及推荐的保存条件。此外，本单元还介绍了制剂的保质期，包括短期、中期和长期保存后复苏冻干的食菌蛭弧菌菌液至活性培养物所需的时间。© 2017约翰威立国际出版公司
