a Area of Plant Physiology, Department of Biology of Organism and Systems , University of Oviedo , Oviedo , Spain.
b Forest and Wood Technology Research Centre (CETEMAS), Sustainable Forest Management Area, Pumarabule , 33936 , Asturias , Spain.
Mycologia. 2017;109(2):350-358. doi: 10.1080/00275514.2017.1323465. Epub 2017 May 2.
The use of spores in applications of ectomycorrhizal fungi requires information regarding spore viability and germination, especially in genera such as Rhizopogon with high rates of spore dormancy. The authors developed a protocol to assess spore viability of Rhizopogon roseolus using four vital stains to quantify spore viability and germination and to optimize storage procedures. They showed that propidium iodide is an excellent stain for quantifying nonviable spores. Observing red fluorescent intravacuolar structures following staining with 2-chloro-4-(2,3-dihydro-3-methyl-(benzo-1,3-thiazol-2-yl)-methylidene)-1-phenylquinolinium iodide (FUN-1) can help identify viable spores that are activated. At 6 mo and 1 y, the spores kept in a water suspension survived better than those left within intact, dry gasterocarps. Our work highlights the importance of temperature, nutrients, and vitamins for maturation and germination of spores of R. roseolus during 1 y of storage.
在外生菌根真菌的应用中使用孢子需要有关孢子活力和萌发的信息，特别是在休眠率较高的 Rhizopogon 等属中。作者开发了一种使用四种活染剂来定量评估 Rhizopogon roseolus 孢子活力和萌发的方法，并优化了储存程序。他们表明碘化丙啶是定量非存活孢子的极好染色剂。用 2-氯-4-（2,3-二氢-3-甲基-（苯并-1,3-噻唑-2-基）亚甲基）-1-苯基喹啉翁碘化物（FUN-1）染色后观察到红色荧光胞内结构有助于识别已激活的存活孢子。在 6 个月和 1 年时，保存在水悬浮液中的孢子比保留在完整干燥的子实体中的孢子存活得更好。我们的工作强调了在 1 年的储存过程中，温度、营养和维生素对 R. roseolus 孢子的成熟和萌发的重要性。
