Nediani Miriam T, García Luis, Saavedra Lucila, Martínez Sandra, López Alzogaray Soledad, Fadda Silvina
Departamento de Ciencias de los Alimentos, Facultad de Agronomía y Agroindustrias, Universidad Nacional de Santiago del Estero. Av. Belgrano 1912, G4200 Santiago del Estero, Argentina.
Centro de Referencia de BacteriasLácticas (CERELA-CONICET), Chacabuco 145, T4000ILC San Miguel de Tucumán, Argentina.
Microorganisms. 2017 May 17;5(2):26. doi: 10.3390/microorganisms5020026.
Quality and safety are important challenges in traditional fermented sausage technology. Consequently, the development of a tailored starter culture based on indigenous microbiota constitutes an interesting alternative. In the present study, spontaneously fermented goat meat sausages were created and analyzed using a physicochemical and microbiological approach. Thereafter 170 lactic acid bacteria (LAB) strains were isolated and preliminary characterized by phenotypic assays. The hygienic and technological properties, and growth and fermentative potential of isolates using a goat-meat-based culture medium were evaluated. All strains proved to have bioprotective features due to their acidogenic metabolism. Almost all grew optimally in meat environments. LAB isolates presented proteolytic activity against meat proteins and enriched amino acid contents of the goat-meat-based model. The most efficient strains were four different isolates, as identified by genotyping and RAPD analysis. strains are proposed as optimal candidates to improve the production of fermented goat meat sausages, creating a new added-value fermented product.
质量和安全是传统发酵香肠技术面临的重要挑战。因此,基于本土微生物群开发定制的发酵剂构成了一种有趣的选择。在本研究中,采用物理化学和微生物学方法制作并分析了自发发酵的山羊肉香肠。此后,分离出170株乳酸菌(LAB)菌株,并通过表型分析进行初步鉴定。评估了使用基于山羊肉的培养基的分离株的卫生和技术特性以及生长和发酵潜力。由于其产酸代谢,所有菌株都具有生物保护特性。几乎所有菌株在肉类环境中生长最佳。LAB分离株对肉蛋白具有蛋白水解活性,并提高了基于山羊肉的模型中的氨基酸含量。通过基因分型和RAPD分析鉴定出最有效的菌株是四种不同的分离株。这些菌株被认为是改善发酵山羊肉香肠生产的最佳候选菌株,可创造一种新的增值发酵产品。