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用于维护外周静脉导管的设备的微生物学检测

Microbiological testing of devices used in maintaining peripheral venous catheters.

作者信息

Rossini Fernanda de Paula, Andrade Denise de, Santos Lissandra Chaves de Sousa, Ferreira Adriano Menis, Tieppo Caroline, Watanabe Evandro

机构信息

PhD, RN, Hospital das Clínicas, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.

PhD, Associate Professor, Escola de Enfermagem de Ribeirão Preto, Universidade de São Paulo, WHO Collaborating Centre for Nursing Research Development, Ribeirão Preto, SP, Brazil.

出版信息

Rev Lat Am Enfermagem. 2017 May 15;25:e2887. doi: 10.1590/1518-8345.1528.2887.

DOI:10.1590/1518-8345.1528.2887
PMID:28513768
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5465976/
Abstract

OBJECTIVE

to evaluate the use of peripheral venous catheters based on microbiological analysis of devices (dressing and three-way stopcocks) and thus contribute to the prevention and infection control.

METHODS

this was a prospective study of microbiological analysis of 30 three-way stopcocks (external surfaces and lumens) and 30 dressing used in maintaining the peripheral venous catheters of hospitalized adult patients.

RESULTS

all external surfaces, 40% of lumens, and 86.7% of dressing presented bacterial growth. The main species isolated in the lumen were 50% coagulase-negative Staphylococcus, 14.3% Staphylococcus aureus, and 14.3% Pseudomonas aeruginosa. Fifty nine percent of multidrug-resistant bacteria were isolated of the three-way stopcocks, 42% of the lumens, and 44% of the dressing with a predominance of coagulase-negative Staphylococcus resistant to methicillin. Besides, 18% gram-negative bacteria with resistance to carbapenems were identified from multidrug-resistant bacteria on the external surfaces of the three-way stopcocks.

CONCLUSION

it is important to emphasize the isolation of coagulase-negative Staphylococcus and gram-negative bacteria resistant to methicillin and carbapenems in samples of devices, respectively, which reinforces the importance of nursing care in the maintenance of the biologically safe environment as well as prevention and infection control practices.

摘要

目的

基于对设备(敷料和三通开关)的微生物学分析评估外周静脉导管的使用情况,从而有助于预防和控制感染。

方法

这是一项前瞻性研究,对30个三通开关(外表面和管腔)以及30个用于维持住院成年患者外周静脉导管的敷料进行微生物学分析。

结果

所有外表面、40%的管腔以及86.7%的敷料均出现细菌生长。管腔中分离出的主要菌种为50%凝固酶阴性葡萄球菌、14.3%金黄色葡萄球菌和14.3%铜绿假单胞菌。59%的多重耐药菌从三通开关中分离出,42%从管腔中分离出,44%从敷料中分离出,以耐甲氧西林凝固酶阴性葡萄球菌为主。此外,在三通开关外表面的多重耐药菌中鉴定出18%对碳青霉烯类耐药的革兰阴性菌。

结论

分别强调在设备样本中分离凝固酶阴性葡萄球菌以及对甲氧西林和碳青霉烯类耐药的革兰阴性菌很重要,这强化了护理在维持生物安全环境以及预防和控制感染措施中的重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baee/5465976/afee0194d7bf/0104-1169-rlae-25-e2887-gf1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baee/5465976/afee0194d7bf/0104-1169-rlae-25-e2887-gf1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baee/5465976/afee0194d7bf/0104-1169-rlae-25-e2887-gf1.jpg

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