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从生乳、奶酪、碎肉和鸡肉样本中分离出的菌株的分子分型及药敏性

Molecular Typing and Antimicrobial Susceptibility of Strains Isolated from Raw Milk, Cheese, Minced Meat, and Chicken Meat Samples.

作者信息

Can Hayriye Yeşim, Elmalı Mehmet, Karagöz Alper

机构信息

Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mustafa Kemal University, Hatay 31000, Turkey.

Microbiology Reference Laboratories Department, Public Health Institutes of Turkey, Ankara 06100, Turkey.

出版信息

Korean J Food Sci Anim Resour. 2017;37(2):175-180. doi: 10.5851/kosfa.2017.37.2.175. Epub 2017 Apr 30.

Abstract

The objectives of this study were: i) to detect the presence of and methicillin-resistant (MRSA) in raw milk, cheese, beef minced meat, and chicken meat samples; ii) to evaluate the antimicrobial susceptibility of the isolates; and iii) to determine clonal relation among the isolates by using pulsed-field gel electrophoresis (PFGE) method. Therefore, a total of 160 food samples were randomly collected between August 2014 and May 2015 in Hatay province, located in the southern Turkey. Twenty (12.5%) of the samples were found to be contaminated with . A total of 40 isolates from the 20 positive samples were confirmed to be by multiplex PCR based on 16S rRNA and gene. The gene was not detected in any of the strains. In the present study, 39 out of 40 (97.5%) isolates were found to be resistant to one or more antibiotics. All of isolates were susceptible to gentamicin, oxacillin, and vancomycin. The highest resistance rate was detected in penicillin (95%) and ampicillin (92.5%), followed by tetracycline (30%), erythromycin (20%), ciprofloxacin (12.5%). Nine major patterns were determined by PFGE. In 6 of these patterns, thirty-six strains (90%) had identical PFGE profiles.

摘要

本研究的目的是

i)检测生牛奶、奶酪、牛肉末和鸡肉样本中金黄色葡萄球菌及耐甲氧西林金黄色葡萄球菌(MRSA)的存在;ii)评估分离株的抗菌药敏性;iii)使用脉冲场凝胶电泳(PFGE)方法确定分离株之间的克隆关系。因此,2014年8月至2015年5月期间,在土耳其南部的哈塔伊省随机收集了总共160份食品样本。发现其中20份(12.5%)样本被金黄色葡萄球菌污染。基于16S rRNA和mecA基因的多重PCR确认,从20份阳性样本中分离出的总共40株菌株为金黄色葡萄球菌。在任何金黄色葡萄球菌菌株中均未检测到mecA基因。在本研究中,40株分离株中有39株(97.5%)对一种或多种抗生素耐药。所有分离株对庆大霉素、苯唑西林和万古霉素敏感。青霉素(95%)和氨苄西林(92.5%)的耐药率最高,其次是四环素(30%)、红霉素(20%)、环丙沙星(12.5%)。通过PFGE确定了9种主要模式。在其中6种模式中,36株菌株(90%)具有相同的PFGE图谱。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e73/5434204/c3287094a783/kosfa-37-175-f001.jpg

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