不依赖阳离子的甘露糖6-磷酸受体含有共价结合的脂肪酸。

Cation-independent mannose 6-phosphate receptor contains covalently bound fatty acid.

作者信息

Westcott K R, Rome L H

机构信息

Department of Biological Chemistry, UCLA School of Medicine 90024.

出版信息

J Cell Biochem. 1988 Sep;38(1):23-33. doi: 10.1002/jcb.240380104.

DOI:10.1002/jcb.240380104

PMID:2851594

Abstract

The cation-independent mannose 6-phosphate receptor (215,000 daltons) was isolated from embryonic bovine tracheal cells and embryonic human skin fibroblasts labelled with [3H]palmitic acid. The tritium label was detected in the protein upon fluorographic analysis of SDS-polyacrylamide gels of the purified receptor. The label was not sensitive to hydroxylamine, methanolic KOH, or beta-mercaptoethanol, but labelled fatty acid was recovered from the protein by acidic methanolysis. Labelled receptor protein could not be isolated from cells grown in the presence of [3H]myristic acid. The results suggest the presence of amide-linked palmitic acid in the structure of the cation-independent mannose 6-phosphate receptor.

摘要

从用[3H]棕榈酸标记的胚胎牛气管细胞和胚胎人皮肤成纤维细胞中分离出不依赖阳离子的甘露糖6-磷酸受体（215,000道尔顿）。对纯化受体的SDS-聚丙烯酰胺凝胶进行荧光分析时，在蛋白质中检测到了氚标记。该标记对羟胺、甲醇氢氧化钾或β-巯基乙醇不敏感，但通过酸性甲醇解从蛋白质中回收了标记的脂肪酸。在[3H]肉豆蔻酸存在下生长的细胞中无法分离出标记的受体蛋白。结果表明，在不依赖阳离子的甘露糖6-磷酸受体结构中存在酰胺连接的棕榈酸。