快速、高灵敏检测小型实验动物血清和脑脊液中抗病毒抗体的简易方法。
Simple method for rapid and highly sensitive detection of antiviral-antibodies in serum and cerebrospinal fluid of small laboratory animals.
作者信息
Sigmund M, Einberger H, Neubert W J
机构信息
Max-Planck-Institut für Biochemie, Abteilung für Virusforschung, Martinsried, F.R.G.
出版信息
J Virol Methods. 1988 Dec;22(2-3):231-8. doi: 10.1016/0166-0934(88)90105-x.
A technique is described for the small-scale determination of antibodies in blood and liquor. Small sample volumes of about 3 microliter blood and 15 microliter liquor are sufficient to detect even low antibody titers against different proteins. Thus, for studying the timecourse of antibody titers, repeated examinations of small laboratory animals are possible. The method includes the highly reproducible separation of the antigens in a 45 X 35 X 0.5 mm polyacrylamide gel (6.5%) using the Phast-system (Pharmacia), followed by electrotransfer and immunostaining. The whole procedure takes less than five hours.
本文描述了一种用于小规模测定血液和脑脊液中抗体的技术。仅约3微升血液和15微升脑脊液的小样本量就足以检测出针对不同蛋白质的低抗体滴度。因此，对于研究抗体滴度的时间进程，对小型实验动物进行重复检测是可行的。该方法包括使用Phast系统（Pharmacia公司）在45×35×0.5毫米的聚丙烯酰胺凝胶（6.5%）中高度可重复地分离抗原，随后进行电转移和免疫染色。整个过程耗时不到五小时。
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